Protein Info for BBR_RS20200 in Bifidobacterium breve UCC2003

Annotation: alpha-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 771 PF16875: Glyco_hydro_36N" amino acids 41 to 303 (263 residues), 118.7 bits, see alignment E=4.8e-38 PF02065: Melibiase" amino acids 314 to 655 (342 residues), 477.9 bits, see alignment E=2.6e-147 PF16874: Glyco_hydro_36C" amino acids 675 to 762 (88 residues), 30.1 bits, see alignment E=8.1e-11

Best Hits

KEGG orthology group: K07407, alpha-galactosidase [EC: 3.2.1.22] (inferred from 91% identity to bln:Blon_2460)

Predicted SEED Role

"Alpha-galactosidase (EC 3.2.1.22)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Melibiose Utilization (EC 3.2.1.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (771 amino acids)

>BBR_RS20200 alpha-galactosidase (Bifidobacterium breve UCC2003)
MAQNTATFTGTAADGTALTAIYLVQPDANVAIGLVAAGDDLPRIIHWGRPLSNPETLLAA
YDALKPQRVSGALDDTAWPSILPTQAESWIGEQRVVLRRDGIELFPKFTVTGMKFDGVVA
ASLDAVSGDSYTDVTGRARTTGPANVPGVAITARDEEQGVELEWHLELLPGGLARQKAIV
TNLFGAEAGAEAPLEIGKIELGFPLPESASEILTTTGHHLRERSPQRQPLTIGRFEKPQL
AGRPDFDASLLLTAGVPGFGFEHGEAYSVHVGWSGNSVLSAERLPYTQGVIGGGELLFGG
EVTLDDAAGEGQSSYETPWLFGSFGDGLNEIAARFHSYVRSLHPRLFSHGRPVILNTWEA
VYFDHNFDTLKALADKAAASGVERFVVDDGWFGSRRDDTSGLGDWQISQDVWPDGPKSLK
ALADYVHAKGMEFGLWFEPEMVNPDSDVARNHPDWILSPTAGRLPLQGRTQQVLDLTNPE
AFDYIYSCMDQLVGELGIDYIKWDHNKLVTEPGSRRSGRPAVHAQTIAVYEIFRGLKTAH
PGLEIESCSSGGGRVDLGILEYADRIWVSDCVDPVERADIQRYTSLLVPPAMMGEHVGAS
PAHSTRRETSQELRMAMAFFGHMGIEWNLLKEPDDALAKLAVWVAEFKKHRDWFAIDTCV
HADSSDPAVRLDGMVMPNRNAAIYRFAQLTTSQTYPAAPVHLPGLDPKRKYRISPLDPSL
DLEGLTNGQSTLGWWNEEGVVLTGEALQRYGIRPPSLHPQQAVLFKAVALS