Protein Info for BBR_RS19905 in Bifidobacterium breve UCC2003

Annotation: methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 621 PF09334: tRNA-synt_1g" amino acids 4 to 410 (407 residues), 502.1 bits, see alignment E=2.4e-154 TIGR00398: methionine--tRNA ligase" amino acids 5 to 543 (539 residues), 483.3 bits, see alignment E=5.1e-149 PF00133: tRNA-synt_1" amino acids 9 to 254 (246 residues), 51 bits, see alignment E=2.5e-17 PF08264: Anticodon_1" amino acids 433 to 535 (103 residues), 31.1 bits, see alignment E=5.1e-11

Best Hits

Swiss-Prot: 99% identical to SYM_BIFLS: Methionine--tRNA ligase (metG) from Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 99% identity to bln:Blon_2426)

Predicted SEED Role

"Methionyl-tRNA synthetase (EC 6.1.1.10)" (EC 6.1.1.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (621 amino acids)

>BBR_RS19905 methionine--tRNA ligase (Bifidobacterium breve UCC2003)
MSHVLVNVAWPYANGPRHIGHVAGFGVPSDVYARYERMKGNDVLMVSGTDEHGTPILVEA
EKEGLTAQELANRYNRVIAKDLCDLGLSYDLFTRTTTGNHEHVVQEMFKQCLENGYIYKG
TQQVAISPSTGRTLPDRYIEGECPICHAEGARGDQCDACGNELDPDDLINPVSKINGETP
RFEQTEHYFLDLPALAEANKAWLETRKGWRTNVINFSLGLFKEVKPRAITRDIDWGIPVP
VKGWIDNPNKKLYVWFDAVIGYLSASIEWARRQGDPEKWREWWNDPKCPAYYFMGKDNIT
FHSQIWPSEMLAYNGKGSKGGETGPMGPLNLPEQVVASEFMTMEGKKFSSSRGIVIYVKD
ILARYPVDAVRYYISVAGPESSDSDFTWAEFVRHNNEELASSWGNLVNRVANLINKNFGE
IPPLDEDSMTNEDRGLLEETSAAFDVVGSSIETHHQKHALSEAMRVVGDINKYISATEPW
KIKDDQARLGTVLHVAAQAVADANHLLAPFLPHSAQKVWEALGGAGTFSPLPELKEVEDL
DKPGFTYPIITGNYELGVNVHPWKSEAIEVGAVVPKPTPIFAKIPTEAVKEELARFDEAL
AARRAAEAERLAAEKAKLAAE