Protein Info for BBR_RS19645 in Bifidobacterium breve UCC2003

Annotation: RNA degradosome polyphosphate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 745 TIGR03705: polyphosphate kinase 1" amino acids 43 to 735 (693 residues), 836.6 bits, see alignment E=8.2e-256 PF13089: PP_kinase_N" amino acids 44 to 149 (106 residues), 129.3 bits, see alignment E=1.8e-41 PF02503: PP_kinase" amino acids 159 to 343 (185 residues), 203.5 bits, see alignment E=5.8e-64 PF17941: PP_kinase_C_1" amino acids 374 to 539 (166 residues), 249.4 bits, see alignment E=3.4e-78 PF13090: PP_kinase_C" amino acids 547 to 721 (175 residues), 190.3 bits, see alignment E=4.9e-60

Best Hits

Swiss-Prot: 62% identical to PPK1_STRGR: Polyphosphate kinase (Fragment) (ppk) from Streptomyces griseus

KEGG orthology group: K00937, polyphosphate kinase [EC: 2.7.4.1] (inferred from 99% identity to bln:Blon_2328)

Predicted SEED Role

"Polyphosphate kinase (EC 2.7.4.1)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism or Purine conversions (EC 2.7.4.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.4.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (745 amino acids)

>BBR_RS19645 RNA degradosome polyphosphate kinase (Bifidobacterium breve UCC2003)
MAQIFDAPSKAILRSQIAEHIAENDNNDRRVDQEGETPLPKDRFFDRELSWLKFNQRVLE
CAENEDMPLLERANFAAIFASNLDEFFMVRVAGLKRRIDSGIAVPSAAGLSPRQQLRAIS
ETAHRLQNEHAHYAIDTILPELEKEHIVLLTWDKLTSAEQERLSRYYRQQVFPVLTPLAV
DPAHPFPYISGGSINLAVIVENPASGKSHFARVKIPGNLPRLVPVDDMTDEESKDERYGF
IAMEKLIAAHLESLFPGMIIKEARSFRVTRNEDIDVEEDDAENLLNAMEKELLRRRFGPP
IRLEITDTTSPFLSQLLADQLGVSQDEVYRLPSPLDLTVLFELGGVDRPDLKNRPFVPTT
NRQIAEVESSRAQDIFAAIRERDILLHHPYDSFSTSVQAFLAQAAADPKVLAIKQTLYRT
SSNSPIIDALIDAAHAGKQVLALVEIKARFDEDANIAWARKLERAGVHVVYGIVGLKTHC
KLIEVVRQEADGLRRYCHVGTGNYNPKTARLYTDLGLLTCDPVVGQDLTRLFNQLSGYAP
KSSFHRLLVAPRTVRTGLIQRIRREEDAARAGKEAWIKIKVNSIVDEKTIDALYRASQAG
VKIDIVERGICALKPGVPGLSENIRVRSILGRFLEHSRIYAFCNADGPQIGEGPASGPEV
YIGSADLMHRNLDRRVEALVRVTAPEQIDELIRYVDLQMADSTMSWHMQPDGTYVLHTKD
DEGRPLVDSQEYLIRKHQRRPSSHN