Protein Info for BBR_RS19630 in Bifidobacterium breve UCC2003

Annotation: 3-isopropylmalate dehydratase large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00170: 3-isopropylmalate dehydratase, large subunit
  • amino acids 1 to 460 (460 residues), 728.4 bits, E=1.6e-223
• PF00330: Aconitase
  • amino acids 7 to 459 (453 residues), 587.5 bits, E=1.1e-180

Best Hits

Swiss-Prot: 98% identical to LEUC_BIFLO: 3-isopropylmalate dehydratase large subunit (leuC) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K01703, 3-isopropylmalate/(R)-2-methylmalate dehydratase large subunit [EC: 4.2.1.33 4.2.1.35] (inferred from 97% identity to bln:Blon_2325)

MetaCyc: 60% identical to 3-isopropylmalate dehydratase subunit LeuC (Escherichia coli K-12 substr. MG1655)
3-isopropylmalate dehydratase. [EC: 4.2.1.33]

Predicted SEED Role

"3-isopropylmalate dehydratase large subunit (EC 4.2.1.33)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 4.2.1.33)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• L-leucine biosynthesis (6/6 steps found)
• 3-methylbutanol biosynthesis (engineered) (6/7 steps found)
• L-isoleucine biosynthesis II (6/8 steps found)
• 2,5-xylenol and 3,5-xylenol degradation (1/13 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• C5-Branched dibasic acid metabolism
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.33, 4.2.1.35

Use Curated BLAST to search for 4.2.1.33 or 4.2.1.35

Protein Sequence (467 amino acids)

>BBR_RS19630 3-isopropylmalate dehydratase large subunit (Bifidobacterium breve UCC2003)
MGTTLAEKVWADHLVRRGSDGAPDLLYIDLMLMHEVTSPQAFEGLRLAGRKPRHVDQLIA
TEDHNTPTVDIDRPNPDETSALQLSTLEKNCKEFGVRLHPLGDADQGIVHAFAPILGLTQ
PGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVMATQTLSLKPFKTMAVNVNGKLPADAT
AKDIILAIIAKIGTGGGQGHVIEYRGEAIRNLTMDERMTVCNMSIEAGARAGMIAPDETT
FEYLKGRPHAPEGEMWDRAVEYWKTLKTDDDAMFDKVVDIDATKLGPFVTWGTNPGQGLP
ITANVPEPDKIADATKRAAAERAITYMGLKPGMPIKDIAVDTVFIGSCTNGRIDDLRQAA
AIMKGHHKAENIHRVLVVPASSRVRLQAEKEGLDKVFKDFGAEWRNAGCSMCLGMNPDKL
VPNERSISTSNRNFEGRQGKGSRTHLASPAVAAATAIRGTISSPADL