Protein Info for BBR_RS19560 in Bifidobacterium breve UCC2003

Annotation: arginine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 620 PF03485: Arg_tRNA_synt_N" amino acids 14 to 98 (85 residues), 76.9 bits, see alignment E=3.8e-25 TIGR00456: arginine--tRNA ligase" amino acids 37 to 561 (525 residues), 339.3 bits, see alignment E=1.9e-105 PF00750: tRNA-synt_1d" amino acids 136 to 441 (306 residues), 69.4 bits, see alignment E=7.1e-23 PF00133: tRNA-synt_1" amino acids 371 to 463 (93 residues), 25.5 bits, see alignment E=1.4e-09 PF05746: DALR_1" amino acids 480 to 620 (141 residues), 99.2 bits, see alignment E=4.1e-32

Best Hits

Swiss-Prot: 96% identical to SYR_BIFLD: Arginine--tRNA ligase (argS) from Bifidobacterium longum (strain DJO10A)

KEGG orthology group: K01887, arginyl-tRNA synthetase [EC: 6.1.1.19] (inferred from 96% identity to blj:BLD_1605)

Predicted SEED Role

"Arginyl-tRNA synthetase (EC 6.1.1.19)" (EC 6.1.1.19)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (620 amino acids)

>BBR_RS19560 arginine--tRNA ligase (Bifidobacterium breve UCC2003)
MSPEALSELISNIAHNLVAAGQAGQLTDELIPPIDKLAVMRPKDRAYGDWASNIAMQLAK
KAGMKPRDLAEPFAAALAEADGIAKVEVAGPGFINITLDSASAAAVVDTVLAAAPAVDGD
KHLNKVNEYGRNAHLGGQTLNLEFVSANPTGPIHIGGTRWAAVGDAMARVLEANGAKVVR
EYYFNDHGEQINRFAKSLVAAWAEANKLGGAGYQTETPCDGYKGAYINEIAARVEAEAKA
DGVNLTALAHQDQGLNDDGEPLGEDDTEVREEFRKRAVPMMFDEIQKSMKDFRVNFDVWF
HENSLYADKKVEAAIEELKSHGDIYDKDGATWFESTKHGDDKDRVIIKSNGEFAYFAADI
AYYWDKRHRAKNPADVAIYMLGADHHGYIGRMMAMCAAFGDEPGKNMQILIGQLANVMKD
GKPVRMSKRAGNVVTIDDLVSVVGVDAARYSLARSDYNQNFDIDLALLASHTNDNPVYYV
QYAHARSKNVDRNAAAAGISYEGADLSLLDTEADGEVLAALAQFPSVLATAADDRQPHKV
ARYLEELAATYHKWYNVERVVPMALTDPETRGDDEARKALEIAKNPEPARAAARLKLNDA
VQQVIANGLDLLGVTAPEKM