Protein Info for BBR_RS19550 in Bifidobacterium breve UCC2003

Annotation: PTS N-acetylglucosamine transporter subunit IIABC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 167 PF00358: PTS_EIIA_1" amino acids 19 to 141 (123 residues), 163.5 bits, see alignment E=9e-53 TIGR00830: PTS system, glucose subfamily, IIA component" amino acids 20 to 140 (121 residues), 146.8 bits, see alignment E=1.4e-47

Best Hits

Swiss-Prot: 41% identical to PTGA_BACHD: PTS system glucose-specific EIIA component (crr) from Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125)

KEGG orthology group: None (inferred from 67% identity to bbi:BBIF_0261)

MetaCyc: 37% identical to Enzyme IIAGlc (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-168 [EC: 2.7.1.201]; TRANS-RXN-157 [EC: 2.7.1.201, 2.7.1.199]; RXN0-17 [EC: 2.7.1.201, 2.7.1.199, 2.7.1.192]

Predicted SEED Role

"PTS system, maltose and glucose-specific IIC component (EC 2.7.1.69) / PTS system, maltose and glucose-specific IIB component (EC 2.7.1.69) / PTS system, maltose and glucose-specific IIA component (EC 2.7.1.69)" in subsystem Maltose and Maltodextrin Utilization (EC 2.7.1.69)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.192 or 2.7.1.199 or 2.7.1.201 or 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (167 amino acids)

>BBR_RS19550 PTS N-acetylglucosamine transporter subunit IIABC (Bifidobacterium breve UCC2003)
MTAAPAAAPKPAGTASTATVLAPVDGTIEPIDQVPDETFAEKLLGDGFAVIPKSDTIVAP
VSGKVSTIAQAKHAVGITTDAGLEVLVHIGIDTVQLNGDPFTVKVTEGRQITAGDPIVEV
DWNAVRAAGKATDVIVVFTNPALVDALAITAAGAVTSGSPVGTVTTK