Protein Info for BBR_RS19445 in Bifidobacterium breve UCC2003

Annotation: glutamate 5-kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00696: AA_kinase
  • amino acids 18 to 245 (228 residues), 130.9 bits, E=6.3e-42
• TIGR01027: glutamate 5-kinase
  • amino acids 18 to 375 (358 residues), 400.2 bits, E=4.6e-124
• PF01472: PUA
  • amino acids 286 to 360 (75 residues), 67 bits, E=1.2e-22

Best Hits

Swiss-Prot: 96% identical to PROB_BIFLO: Glutamate 5-kinase (proB) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 96% identity to blb:BBMN68_1543)

MetaCyc: 42% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-ornithine biosynthesis II (2/3 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• L-N^δ-acetylornithine biosynthesis (3/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Protein Sequence (377 amino acids)

>BBR_RS19445 glutamate 5-kinase (Bifidobacterium breve UCC2003)
MSTPSQAEVRRMIAAAGTIVVKVGSSSLTQPSGHLDPDKLDALTAALAQVRLMGARVVLV
SSGAIAAGFGPLGFDERPADVATQQATAAVGQGLLMARYEAAFGRFGIRVGQILITAEDT
IRATQYRNVERTLDRLLDLGVVPIINENDSLASNEIRFGDNDRLSALVANLVRAEALVLL
TDVDALYTAPPAQPGSRRVEYVPNVIDALGDIQVSGSGSKVGTGGMVTKLEAARVAAVSG
IPTVLTCASNAGPAMMGDPVGTVFAPVKARGSSRRLWIGFAAEPRGTIVVDAGAAKAIRG
GRASLLAAGALDVHGDFSAGDPVWIDDESGTHLARGLAGFDSEEIPQTLGRNTAQLKRFL
GDQYAHPLVHRDNLVLV