Protein Info for BBR_RS19220 in Bifidobacterium breve UCC2003

Annotation: glycoside hydrolase family 65 protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 777 PF03636: Glyco_hydro_65N" amino acids 23 to 263 (241 residues), 175.7 bits, see alignment E=1.9e-55 PF03632: Glyco_hydro_65m" amino acids 322 to 698 (377 residues), 386.6 bits, see alignment E=1.8e-119 PF03633: Glyco_hydro_65C" amino acids 710 to 770 (61 residues), 44 bits, see alignment 2.2e-15

Best Hits

KEGG orthology group: K10231, kojibiose phosphorylase [EC: 2.4.1.230] (inferred from 98% identity to blf:BLIF_0278)

Predicted SEED Role

"Maltose phosphorylase (EC 2.4.1.8) / Trehalose phosphorylase (EC 2.4.1.64)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis or Trehalose Uptake and Utilization (EC 2.4.1.64, EC 2.4.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.230 or 2.4.1.64 or 2.4.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (777 amino acids)

>BBR_RS19220 glycoside hydrolase family 65 protein (Bifidobacterium breve UCC2003)
MIRDSFSTALGFELPTDGWMVSEDAYEPDRNLQYESLFALSNGYLGIRGSHEEGNKPTLP
YLYVNGVFDKSETFMKELATLPNWLGIRLYVEKQLIGVDECEILEYRRALDLKHAALVKR
VRLRDPKGRETLIEGVRFVSRAHAHRMAVRLWVTPLNYSGLIEVEQITDGSIINFYDAPR
FKVKHTVVTANETLGENGVYMEVATRDENLHVGVGSHLEGTCRDRDVLGNRVFHAFGEQG
VEFCDFRADEGDSVVLTKYVAMLDGRDTPADELHATTRREVEDFVAEGFEREFEAHADIY
RKMWGQANIDIEGDPELDRAIRLNVFHLMSTANEHDDRVNVGAKLLTGEEYGGHAFWDTE
LFMLPFFAYVFPQTARHLESYRYRLLGAAEANAAKNGFQGAQYPWESADDGSEQCPDWTI
EPDGTCYRCYVAVYEHHVTAAVAFGIANYAIITGDTSFLYGRGAEILTQTARFWASRCEW
KEDAGRYEITQVTGPDEWHEPVDNNTYTNYLARWNMRYVIALVDRMQREHPDNYAALIAA
TGLTDAELASWDAIQSKIYLPRKEGADLLEQFEGYFDLEPVTIEEYDKNDWPVRPAALQQ
FKTSETQIIKQPDVVMLLHLLGNEFSQQEIETNYHYYEKRTLHGSSLSPSIYAIMGLKVG
DDSKAYRYLRRAAMLDLLNLQHNTREGIHAANAGGIWQTVVFGFAGVNMDDDGVLSITPH
MPHEWSSLTFRLHNRESWLEIRVDATNGVDVEVLDGPSAILRISGETVTVPGAKDVS