Protein Info for BBR_RS18795 in Bifidobacterium breve UCC2003

Annotation: tRNA pseudouridine(38-40) synthase TruA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00071: tRNA pseudouridine(38-40) synthase
  • amino acids 5 to 278 (274 residues), 151.8 bits, E=1.2e-48
• PF01416: PseudoU_synth_1
  • amino acids 162 to 285 (124 residues), 67.2 bits, E=8.4e-23

Best Hits

Swiss-Prot: 89% identical to TRUA_BIFLO: tRNA pseudouridine synthase A (truA) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K06173, tRNA pseudouridine synthase A [EC: 5.4.99.12] (inferred from 89% identity to bln:Blon_2209)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Protein Sequence (303 amino acids)

>BBR_RS18795 tRNA pseudouridine(38-40) synthase TruA (Bifidobacterium breve UCC2003)
MTRLRIDLAYDGGGFYGWAKQPELRTVQGTIEDALHKVLRVPVGDEREPLRLTVAGRTDT
GVHASHQVAHLDVSDETLARCQGHMDVPLTEALARRLKAVLPADIVIHGIAIAPQGFDAR
FSALERTYVYRVADRSSEVDPRLRGCVLRVDESLDLDAMNAAAAMTIGLHDFGSFATPNP
GGTTIREVKTAYWRRVPVASLVPDVLAENEAYRTPSLESGLVVFTIVADAFARNMVRSLV
GSSIKVGSGRKTLEWFADKMANPVREGSSGPIAPQGLTLEHIEYPADDQLAARAEAIRAV
RTL