Protein Info for BBR_RS18660 in Bifidobacterium breve UCC2003

Annotation: 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 TIGR02336: 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase" amino acids 2 to 749 (748 residues), 1322.9 bits, see alignment E=0 PF09508: Lact_bio_phlase" amino acids 4 to 431 (428 residues), 685.6 bits, see alignment E=3.2e-210 PF17385: LBP_M" amino acids 439 to 691 (253 residues), 289.3 bits, see alignment E=2.9e-90 PF17386: LBP_C" amino acids 696 to 748 (53 residues), 72.6 bits, see alignment 3e-24

Best Hits

Swiss-Prot: 91% identical to LNPA_BIFL2: 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase (lnpA) from Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)

KEGG orthology group: None (inferred from 92% identity to blf:BLIF_1691)

Predicted SEED Role

"Lacto-N-biose phosphorylase (EC 2.4.1.211)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway (EC 2.4.1.211)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.211

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (750 amino acids)

>BBR_RS18660 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase (Bifidobacterium breve UCC2003)
MSTGRFTLPSEENFAEKTKELAELWGADAIRNSDGTHLDEDVLALGKKIYSAYFPTRAHN
EWITLHMDETPQVYLLTDRILAESDAVDVPLMDGFFEEQLKPNRDADPHRYWEVVDRTTG
EVVPPEQWTLDAGEDTVHVTAAVPMHEYTVSFLAYIIWDPVEMYNHLTNDWGDKEHEIPF
DIYHPATRKFVFDTFARWLKDSPQTDVVRFTTFFYQFTLLFDAKRREKVVDWFGCACTVS
PRALDDFEKEYGYRLRPEDFVDGGAYNSAWRVPRKAQRDWIDFLSGFVRKNVKQLADMSH
AAGKEAMMFLGDQWIGTEPYKDGFDELGLDAVVGSIGDGTTTRMIADIPGVKYTEGRFLP
YFFPDTFYEGNDPSIEGLDNWRKARRAILRSPISRMGYGGYLSLAAKFPKFVDTVTHIAD
EFRDIHDRTDGVAAEGELNVAILNSWGRMRSWMAFTVAHALPNKQTYSYYGILESLSGMR
VNVRFVSFDDILEHGVDPDIDVIINGGPVDTAFTGGDVWANPKLVETLRAWVRDGGALVG
VGEPSSLPRFQAGRFFQLADVLGVDEERFQTLSVDKYFPPVTPEHFITTDVPVDPAARGA
WERAGYRAPLSGCGGGQGIAPLGGIDFGEAVANTYPVSEDVTLLRADNGQVQLAANEYGK
GRGVYISGLPYSAANARLLERALFWASHKEDRYAAWSSSNPECEVAHFPGQGLYCVVNNT
DQPQTTTVTLADGSSETFDLQPSGIAWRND