Protein Info for BBR_RS18650 in Bifidobacterium breve UCC2003

Annotation: UDP-glucose 4-epimerase GalE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04321: RmlD_sub_bind
  • amino acids 3 to 163 (161 residues), 35 bits, E=3.1e-12
• TIGR01179: UDP-glucose 4-epimerase GalE
  • amino acids 4 to 335 (332 residues), 426.7 bits, E=2.5e-132
• PF01370: Epimerase
  • amino acids 4 to 262 (259 residues), 181.7 bits, E=5.8e-57
• PF02719: Polysacc_synt_2
  • amino acids 4 to 126 (123 residues), 41.1 bits, E=4.7e-14
• PF16363: GDP_Man_Dehyd
  • amino acids 5 to 322 (318 residues), 191.4 bits, E=1.1e-59
• PF01073: 3Beta_HSD
  • amino acids 5 to 160 (156 residues), 44.6 bits, E=3.4e-15
• PF07993: NAD_binding_4
  • amino acids 6 to 165 (160 residues), 20.3 bits, E=9.5e-08

Best Hits

Swiss-Prot: 98% identical to GALE_BIFL2: UDP-glucose 4-epimerase (lnpD) from Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 98% identity to blm:BLLJ_1620)

MetaCyc: 59% identical to UDP-glucose 4-epimerase (Bacillus subtilis subtilis 168)
UDP-N-acetylglucosamine 4-epimerase. [EC: 5.1.3.7]; UDP-glucose 4-epimerase. [EC: 5.1.3.7, 5.1.3.2]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• stachyose degradation (7/7 steps found)
• D-galactose detoxification (3/3 steps found)
• UDP-α-D-galactofuranose biosynthesis (2/2 steps found)
• D-galactose degradation I (Leloir pathway) (4/5 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• colanic acid building blocks biosynthesis (4/11 steps found)
• UDP-sugars interconversion (1/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (1/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (13/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (2/18 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.7

Protein Sequence (340 amino acids)

>BBR_RS18650 UDP-glucose 4-epimerase GalE (Bifidobacterium breve UCC2003)
MTTVLVTGGAGFIATHTDIELLNKGYDVISVDNYGNSSPVALERVEQITGKPVKRYDGDV
RDEALMERVFTENNIDWVIHFAGLKAVGESVAKPIEYYDNNLYSTLVLLKVMKKHNVKKI
IFSSSATVYGSPKELPITEETPTGGTTNPYGTSKLFQEQILRDVHVADPSWTIVLLRYFN
PVGAHESGLLGEDPKGIPANLTPYVAKVAVGELKAVQVYGDDYDTPDGTGVRDYIHVVDL
AKGHVAVIDHIDKEGVFVYNLGTGHGYSVLEVIKAYEKAAGHPIPYVIKPRRPGDIAACY
ADASKAAKELDWKAELTIDDMAASSLNWQTKNPNGFRDAE