Protein Info for BBR_RS18600 in Bifidobacterium breve UCC2003

Annotation: lysine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 560 TIGR00499: lysine--tRNA ligase" amino acids 65 to 557 (493 residues), 464.7 bits, see alignment E=1.7e-143 PF01336: tRNA_anti-codon" amino acids 118 to 193 (76 residues), 41.4 bits, see alignment E=1.2e-14 PF00152: tRNA-synt_2" amino acids 210 to 554 (345 residues), 270.2 bits, see alignment E=2.4e-84

Best Hits

Swiss-Prot: 62% identical to SYK_KINRD: Lysine--tRNA ligase (lysS) from Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)

KEGG orthology group: K04567, lysyl-tRNA synthetase, class II [EC: 6.1.1.6] (inferred from 98% identity to blf:BLIF_1679)

Predicted SEED Role

"Lysyl-tRNA synthetase (class II) (EC 6.1.1.6)" (EC 6.1.1.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (560 amino acids)

>BBR_RS18600 lysine--tRNA ligase (Bifidobacterium breve UCC2003)
MAEVVENTENTENETPAVPQMSTVERAEMLLKQDATIREKIKSGATLDEAVDPSNKEFGP
LGHPEQVQMRVAKRAMMLESGIAPYPVHLDVTNTIEEVRAKYDGKLEAGQETEDMVGIAG
RVLFLRNAGGLCFVQLSAGDGTRIQGMISKKEIGADSLKQFKQLVDLGDHLYLKGRVIAS
KTGELSVFATEWAIASKALQPLPALHKDLNEDTRTRKPYIGMIADENIRNMVRNRSKAVA
SLRRTFDAHDFLEVETPMLQTLHGGAAARPFTTHMNAFDLDLYLRIAPELFLKRCLVGGI
DRVFEINRDFRNEGVDATHAPEFTMVEAYQAYGTYDSIGQLVKELVQQTAMDVYGSHKVT
LLDGTEYDFGGEWKTISMYDSLSEALGEEIVPNGGPEHPGTSVEHLGEIADKLGVERDDV
ENHGKLVEHLWEHFYEDKLYEPTFVRDFPVETSPLVKGHRTKPGVVEKWDLYVRGFELAT
GYSELNDPIVQRERFVAQAKDALAGDEEACDIDEDFLEALGVGMPPAGGMGMGIDRLLIA
LTGATIRETITFPLVKPLVG