Protein Info for BBR_RS18375 in Bifidobacterium breve UCC2003

Annotation: ATP-dependent helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 567 PF00580: UvrD-helicase" amino acids 8 to 256 (249 residues), 118.4 bits, see alignment E=7.4e-38 PF13245: AAA_19" amino acids 22 to 249 (228 residues), 53.1 bits, see alignment E=5.6e-18 PF13361: UvrD_C" amino acids 454 to 512 (59 residues), 40.3 bits, see alignment 4.2e-14

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 60% identity to mcu:HMPREF0573_10207)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (567 amino acids)

>BBR_RS18375 ATP-dependent helicase (Bifidobacterium breve UCC2003)
MTSTEYVMTQEQQGIFNYRPLNMVITAPAGCGKTEALAYRAKGLINRYDFAGNGRKLLVV
SFTNQAKDNISERIKKHIGLQTMRQHVTVCNFHGLSARIINAHREMIGLDDEWTVASFDW
IGRFMQSLNCDRKVKMQAREALQKIKLECLTDKEVKAKIQTIYGQVGNLAQIIEKERIKE
KIITYDDQIRTALWILQNKNVAKLYRNHFFAALVDEFQDLTHQQLRLVQALCKDRVTFAG
DLAQGIYSFAGGDARFVFHEITKNTEKQVKLLKSFRSSPAILDAVNSLSPRTGSEHLSAA
FPEHWGNGGLSSYASFNREDAEAEWVVRMCQLILERCPEHRIGIISRTAFRAESVKSTLK
SHNISYADWGNGLFRPETAKILRNICDELSVESCKEPEELMSFIKKRYTAFHGNLSEEHE
DACGWLFDRLAQSDASSIAEVKRCINAKKGNKTIATRKGIHCLTGHAGKGQQFDWVFIVG
LEEGTIPFYKAKSEEAIDEEARVLSVMISRARIGYVATSSSTNPRFWYSRNASSFLRYLQ
HAPNFLFGKDQIELWCAEADWTALSQM