Protein Info for BBR_RS18245 in Bifidobacterium breve UCC2003

Annotation: tRNA (guanosine(46)-N7)-methyltransferase TrmB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 307 TIGR00091: tRNA (guanine-N(7)-)-methyltransferase" amino acids 102 to 242 (141 residues), 119.3 bits, see alignment E=6.9e-39 PF02390: Methyltransf_4" amino acids 105 to 240 (136 residues), 125.2 bits, see alignment E=1e-40

Best Hits

Swiss-Prot: 88% identical to TRMB_BIFLO: tRNA (guanine-N(7)-)-methyltransferase (trmB) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K03439, tRNA (guanine-N7-)-methyltransferase [EC: 2.1.1.33] (inferred from 82% identity to bll:BLJ_1645)

Predicted SEED Role

"tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33)" (EC 2.1.1.33)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.33

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (307 amino acids)

>BBR_RS18245 tRNA (guanosine(46)-N7)-methyltransferase TrmB (Bifidobacterium breve UCC2003)
MSETRNVTPGSSNHSEEHMTDPTTTPTTAGVANATTDDASATHPHPLHKVLSFVRRSGRL
DDRLQRAWDNYADTYLLDITTGNLLDVREGVTLNRNFVAQHWGNDNPLIVEIGTGQGENV
VAAAAAHPETNFLALEVYDPGVAHTLLLAGKQGLPNIRVAQVNAPELFKVTELGTVAEVW
TFFPDPWPKKKHHKRRIVQVGMSDDIHRALVENGLWRIATDIEDYALHVHEVMDHLDGWT
NLGGVTVSLPVEHVGKGNADMAADMPHADFKESERFEGRVLTNFEKKGLAAGRAIHDFTY
QAVPVSR