Protein Info for BBR_RS18190 in Bifidobacterium breve UCC2003

Annotation: tRNA lysidine(34) synthetase TilS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 383 PF01171: ATP_bind_3" amino acids 45 to 215 (171 residues), 127.6 bits, see alignment E=4.9e-41 TIGR02432: tRNA(Ile)-lysidine synthetase" amino acids 45 to 236 (192 residues), 138.7 bits, see alignment E=1.1e-44 PF09179: TilS" amino acids 310 to 369 (60 residues), 27.5 bits, see alignment E=3.2e-10

Best Hits

Swiss-Prot: 68% identical to TILS_BIFLO: tRNA(Ile)-lysidine synthase (tilS) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K04075, tRNA(Ile)-lysidine synthase [EC: 6.3.4.-] (inferred from 68% identity to blo:BL1680)

Predicted SEED Role

"tRNA(Ile)-lysidine synthetase (EC 6.3.4.19)" (EC 6.3.4.19)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.- or 6.3.4.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (383 amino acids)

>BBR_RS18190 tRNA lysidine(34) synthetase TilS (Bifidobacterium breve UCC2003)
MAYSARLRKAIGAVRLALESAGLGMQSPEFARHGEHQAAPDAPLVLVACSGGRDSMALAA
VCRIVCASLGLRCGAVIVDHGLQSGSDRVATLTAERCRDCGLAPVAVRTASVHVQGEGVE
AAAREARYGELCGAARELNAAVVLLAHTLDDQAETVLIGLLRSGGVDALAGMPQVMHRDG
IMFLRPLIGVSRAETTGICEDLNIHYWDDPTNGDDVDGELPRDYPLRSRIRHDLLPAIKR
FAGADVTQHIANGARLARLDKEYLDQQADKTMIEALGSTLKTAESQGTSVAGMTDKSNDL
EREPSLVIAVKPLQSTHEAIRLRAIAHALSRAGISASSAQIEAINRLVVDWHGQGAVALP
SGYSAKRQKHVIRVCQDRAHANR