Protein Info for BBR_RS17900 in Bifidobacterium breve UCC2003

Annotation: glutamate--cysteine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF04107: GCS2
  • amino acids 83 to 354 (272 residues), 106 bits, E=1.2e-34

Best Hits

KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 100% identity to blj:BLD_1932)

Predicted SEED Role

"Similar to Glutamate--cysteine ligase (EC 6.3.2.2)" (EC 6.3.2.2)

MetaCyc Pathways

• glutathione biosynthesis (1/2 steps found)
• homoglutathione biosynthesis (1/2 steps found)
• ophthalmate biosynthesis (1/3 steps found)
• ergothioneine biosynthesis I (bacteria) (3/7 steps found)
• γ-glutamyl cycle (1/6 steps found)

KEGG Metabolic Maps

• Glutathione metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.2

Protein Sequence (425 amino acids)

>BBR_RS17900 glutamate--cysteine ligase (Bifidobacterium breve UCC2003)
MVTPRISYAHLLAKPNPKHVESLLKFFENGRSQRGTGGFGVEIEHLPVHNSDDTAVSYYE
PNGIEALLKRLAPYYDEEKEYWENGHLVGLGRSGVAVSLEPGGQVETSIGILKKPSDLNT
LYSKFRRELDPILDDLDFRLVNYGYQPKSSFADVPVNPKDRYDAMTDYLGRVGQFGPCMM
RCSASTQVSIDYVDERDSIEKLRLGTVIGPILAYFFRNTPYFEGETNPWPLLRQRMWDYL
DFQRTNVLPGLFDDRYGWEDYAIDVLSTPLMFADLTHTPEAVASGASPKELHRPAFRENA
GEVYPDRELNPYEINHIISTHFNDVRLKNFIELRHWDSLPIERAERLTEIVSSLFYVSEH
RERLESYFEGISEEEVFEAKANIQAHGREASPYGQPLDFWKEFLGLEGLLSDIPGDLKHP
DVFQE