Protein Info for BBR_RS17815 in Bifidobacterium breve UCC2003

Annotation: nucleoside hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01156: IU_nuc_hydro
  • amino acids 7 to 316 (310 residues), 330.7 bits, E=5.4e-103

Best Hits

Swiss-Prot: 43% identical to RIHA_ECOBW: Pyrimidine-specific ribonucleoside hydrolase RihA (rihA) from Escherichia coli (strain K12 / MC4100 / BW2952)

KEGG orthology group: None (inferred from 99% identity to blb:BBMN68_1806)

MetaCyc: 43% identical to pyrimidine-specific ribonucleoside hydrolase RihA (Escherichia coli K-12 substr. MG1655)
Uridine nucleosidase. [EC: 3.2.2.3]; 3.2.2.3 [EC: 3.2.2.3]

Predicted SEED Role

"Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1)" in subsystem Purine conversions or Queuosine-Archaeosine Biosynthesis (EC 3.2.2.1)

MetaCyc Pathways

• adenine and adenosine salvage II (2/2 steps found)
• guanine and guanosine salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage II (2/2 steps found)
• pyrimidine ribonucleosides salvage III (2/2 steps found)
• adenosine nucleotides degradation I (5/8 steps found)
• guanosine nucleotides degradation I (2/4 steps found)
• guanosine nucleotides degradation II (2/4 steps found)
• superpathway of pyrimidine ribonucleosides salvage (6/10 steps found)
• superpathway of guanosine nucleotides degradation (plants) (3/6 steps found)
• purine nucleotides degradation I (plants) (7/12 steps found)
• NAD salvage (plants) (6/11 steps found)
• superpathway of purines degradation in plants (7/18 steps found)

KEGG Metabolic Maps

• Nicotinate and nicotinamide metabolism
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.1

Use Curated BLAST to search for 3.2.2.1 or 3.2.2.3

Protein Sequence (327 amino acids)

>BBR_RS17815 nucleoside hydrolase (Bifidobacterium breve UCC2003)
MENKKKVILDCDPGHDDAFGIMLAVQHLDVLGITTIGGNCTLENVTRNAIKVLEVLGAAD
RIGVYPGHSCPMVAPLVTAPQFHGETGLDGPVLPEPTHAAQNKHAVDFIVDTVMNTDDVT
LIATGPLTNIASAINREPRIVERVKELCIMGGSVTYGNWTPAAEFNIFVDPEAASRVFNS
GMHVKMTGINLTRQCELTAKHVAKFREIGTKAANLAADLTDFFIGACEEESELTGATMHD
ACAVTWVIDPSLITAVPMHIDIELHGTLTRGMTVCDYRHLRGTVPAVDLERTPTMGYRGE
EPNAEAALELDFPGFVDLLNSTLENYD