Protein Info for BBR_RS17720 in Bifidobacterium breve UCC2003

Annotation: CRISPR-associated helicase/endonuclease Cas3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 812 TIGR01596: CRISPR-associated endonuclease Cas3-HD" amino acids 24 to 215 (192 residues), 64.4 bits, see alignment E=1.4e-21 PF01966: HD" amino acids 25 to 118 (94 residues), 27.8 bits, see alignment E=4.1e-10 PF04851: ResIII" amino acids 266 to 404 (139 residues), 38.6 bits, see alignment E=1.6e-13 PF00270: DEAD" amino acids 275 to 432 (158 residues), 55.7 bits, see alignment E=8e-19 TIGR01587: CRISPR-associated helicase Cas3" amino acids 277 to 617 (341 residues), 124.2 bits, see alignment E=6.6e-40

Best Hits

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (812 amino acids)

>BBR_RS17720 CRISPR-associated helicase/endonuclease Cas3 (Bifidobacterium breve UCC2003)
MGHALMPKDLLLARKDDDGNTQTLYDHLHNTAKLAAGFEKRSSVTAFAAGLLHDSGKATD
DFKRYLLTGNRKRGEVVHAKQGAFIVDDCAADFPSSAASMITAEVLELAIANHHGHLPDC
LDACGGTAYFRKLTGENKKAEQYHYQEVLSHVSSLNLDINTNFANSVAETNRLLETVDSC
GISPRNTRNSRSFFLGLYAKYIYSRLVDADRVDAANFTAHQPYTSQTPNWEELTRRLENA
ISSFDTSSKISKIRMRISEDCLAASTRDTGIYRLGVPTGGGKTLASLRFALHHAQETGKH
HIIYITPYLSITEQTVQVFRNVLDLDKDDEILLEHYSSVVPDKDDEKEQQRRLAAERWDK
PIIVTTMVQFLETVMSAKATKLRKFHNLADSVIVFDEVQALPTNTINVFNEVVSFLSKIL
GSTILLCSATQPLLEQTDRRNLLLSDKPDLIDPDNDYAEQLRRTNIVASSQGLTLDDFAA
EIYSKALDNGNCLAVVNLKGEAKQLYQLVKELDVDHRFTLVHLSTAMCGAHRKNQLAVLK
KCLPKKTGDSGQPVICISTQLIEAGVDISFSCVIRAMAGLDSILQAAGRCNRNGESQMPR
NVYVYPIKDESGLRYLPEIQLGKEITDQLIHDYPEQDLLSAFMIEEYYRAYLIRYPKLVD
SKSRNIGTMDYSILGGEETVYDLLSFNRRRRGLYCNRVGEQYPYAFAQAFQTVGDSFHVI
PGGTTDVVVHYGQAMELIDQLSGEGDFASKIKILRSLQEYTISLFDYEFKALDKSKSIGE
ANGDFGILVLNEENYSQDFGFLLKAEMPLLMK