Protein Info for BBR_RS17700 in Bifidobacterium breve UCC2003

Annotation: CRISPR-associated protein Cas4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 229 TIGR00372: CRISPR-associated protein Cas4" amino acids 15 to 204 (190 residues), 114 bits, see alignment E=3.6e-37 PF01930: Cas_Cas4" amino acids 16 to 203 (188 residues), 83.9 bits, see alignment E=6.9e-28

Best Hits

KEGG orthology group: K07464, putative RecB family exonuclease (inferred from 71% identity to bad:BAD_1181)

Predicted SEED Role

"CRISPR-associated RecB family exonuclease Cas4"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (229 amino acids)

>BBR_RS17700 CRISPR-associated protein Cas4 (Bifidobacterium breve UCC2003)
MKEPTDYPEDDWLALSGLQHFAFCRRQWALIHIEQQWQDNYLTTAGSLEHDRAHDYSESE
TRGNLLIMRDLRVFSRRLGITGACDVVEFRQSDEGVPLHGRAGTWLPYPVEYKHGVPKQV
DADRLQLCAEAMCLEEMLACSIPEGSLFYQQTKRRERVQFDESLRGQIVAMTSEMHDLFS
RGHTPKVRPSKACRACSLSDICLPKIAKTRSAQQFIEESVQAGVEGVAG