Protein Info for BBR_RS17690 in Bifidobacterium breve UCC2003

Annotation: CRISPR-associated endonuclease Cas2

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 25 50 75 96 PF09827: CRISPR_Cas2" amino acids 1 to 78 (78 residues), 82.7 bits, see alignment E=8.7e-28 TIGR01573: CRISPR-associated endonuclease Cas2" amino acids 1 to 88 (88 residues), 60.6 bits, see alignment E=7e-21

Best Hits

Swiss-Prot: 52% identical to CAS2_CHLTE: CRISPR-associated endoribonuclease Cas2 (cas2) from Chlorobaculum tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)

KEGG orthology group: None (inferred from 71% identity to bad:BAD_1179)

Predicted SEED Role

"CRISPR-associated protein Cas2" in subsystem CRISPRs

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (96 amino acids)

>BBR_RS17690 CRISPR-associated endonuclease Cas2 (Bifidobacterium breve UCC2003)
MMVVVAYDVNTETPDGKRRLRSVAKTCMKYGQRVQNSVFECSVTPSDYLILKHDLAEIMD
EMCDSLRYYNLGSKYASKIEHRGRQRHVPVDGVMML