Protein Info for BBR_RS17485 in Bifidobacterium breve UCC2003

Annotation: uracil-DNA glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 229 PF03167: UDG" amino acids 58 to 217 (160 residues), 49.4 bits, see alignment E=2.8e-17

Best Hits

Swiss-Prot: 92% identical to UNG_BIFLO: Uracil-DNA glycosylase (ung) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K03648, uracil-DNA glycosylase [EC: 3.2.2.-] (inferred from 93% identity to bln:Blon_0691)

Predicted SEED Role

"Uracil-DNA glycosylase, family 1" in subsystem DNA Repair Base Excision

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (229 amino acids)

>BBR_RS17485 uracil-DNA glycosylase (Bifidobacterium breve UCC2003)
MIKPLRELVEPGWANALADVEPTIHRMGDFLREENAAGRPWLPASHNILRAFTIPFDSIK
VLIVGQDPYPTPGHPVGLSFCVAPEVRPLPKSLINIYKELVDDLGVPMPPNGDLTAWTER
GVMLLNRCLTVGVGRPNSHQGKGWEEVTEAAIRALNARVDSNGKPRPLVAILWGRNAQSL
EPLLTNAFIIKSPHPSPLSASRGFFGSKPFSRANQALINMGAEPVDWSL