Protein Info for BBR_RS17265 in Bifidobacterium breve UCC2003

Annotation: beta-fructofuranosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00251: Glyco_hydro_32N
  • amino acids 44 to 354 (311 residues), 300.7 bits, E=1.5e-93
• PF08244: Glyco_hydro_32C
  • amino acids 358 to 510 (153 residues), 100.3 bits, E=1.1e-32

Best Hits

KEGG orthology group: K01193, beta-fructofuranosidase [EC: 3.2.1.26] (inferred from 98% identity to blf:BLIF_1386)

Predicted SEED Role

"Sucrose-6-phosphate hydrolase (EC 3.2.1.B3)" (EC 3.2.1.B3)

MetaCyc Pathways

• sucrose degradation III (sucrose invertase) (4/4 steps found)
• sucrose degradation V (sucrose α-glucosidase) (3/5 steps found)
• inulin degradation (1/3 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.26, 3.2.1.B3

Use Curated BLAST to search for 3.2.1.26 or 3.2.1.B3

Protein Sequence (518 amino acids)

>BBR_RS17265 beta-fructofuranosidase (Bifidobacterium breve UCC2003)
MTDFTPETPVLTPIRDHAAELAKAEAGVAEMAAKRNNRWYPKYHIASNGGWINDPNGLCF
YKGRWHVFYQLHPYGTQWGPMHWGHVSSTDMLNWKREPIMFAPSLEQEKDGVFSGSAVID
DNGDLRFYYTGHRWANGHDNTGGDWQVQMTALPDNDELTSATKQGMIIDCPTDKVDHHYR
DPKVWKTGDTWYMTFGVSSEDKRGQMWLFSSKDMVRWEYERVLFQHPDPDVFMLECPDFF
PIKDKDGNEKWVIGFSAMGSKPSGFMNRNVNNAGYMIGTWEPGGEFKPETEFRLWDCGHN
YYAPQSFNVDGRQIVYGWMSPFVQPIPMEDDGWCGQLTLPREITLDDDGDVVTAPVAEME
GLREDTLDHGSITLDMDGEQVIADDAEAVEIEMTIDLAASTADRAGLKIHATEDGAYTYV
AYDDQIGRVVVDRQAMANGDHGYRAAPLTDAELASGKLDLRVFVDRGSVEVYVNGGHQVL
SSYSYASEGPRAIKLVAEFGNLKVESLKLHHMKSIGLE