Protein Info for BBR_RS17205 in Bifidobacterium breve UCC2003

Annotation: phosphomethylpyrimidine synthase ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 97% identical to THIEC_BIFLO: Thiamine biosynthesis bifunctional protein ThiEC (thiE/thiC) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 98% identity to bll:BLJ_1351)

Predicted SEED Role

"Thiamin biosynthesis protein ThiC / Thiamin-phosphate pyrophosphorylase (EC 2.5.1.3)" in subsystem Thiamin biosynthesis (EC 2.5.1.3)

MetaCyc Pathways

thiamine diphosphate salvage IV (yeast) (6/7 steps found)
thiamine diphosphate salvage II (4/5 steps found)
thiamine diphosphate biosynthesis III (Staphylococcus) (2/3 steps found)
thiamine diphosphate biosynthesis IV (eukaryotes) (2/3 steps found)
thiamine diphosphate salvage V (2/3 steps found)
thiamine diphosphate biosynthesis I (E. coli) (1/2 steps found)
thiamine diphosphate biosynthesis II (Bacillus) (1/2 steps found)
thiamine diphosphate formation from pyrithiamine and oxythiamine (yeast) (5/8 steps found)
superpathway of thiamine diphosphate biosynthesis II (7/11 steps found)
superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (4/7 steps found)
superpathway of thiamine diphosphate biosynthesis I (6/10 steps found)

KEGG Metabolic Maps

Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (917 amino acids)

>BBR_RS17205 phosphomethylpyrimidine synthase ThiC (Bifidobacterium breve UCC2003)
MSNEYPYASMRDSFDLSAYFVVGPEDCKGRPLTDVVDQALHGGATFIQLRAKEADASELT
DMARDIAQIIEDNEKSDSVAFVIDDRADVVWQARRKGIKVDGVHIGQTDMEPREARALLG
DEAIVGLSAETESLVQLINELPDGCIDYIGAGPLHVSTTKPEASVGGNDGSGKTLDAAQI
NTICVASEFPVVVGGGVTAADMAMLAGTKAAGWFAVSAIAGAENPEGATRAMVEGWKAVR
GDKKHGYAPRIVAHAPAADTQAAQEGTAAAGSEDTEKKFTNAKQAKDAQKLAKQQRVDIA
ARGSEQRDKAHIRKTKTVPFTYQYGSYDLEVPYTEIKLSDTPGVGPNPPFHDYSTEGPKC
DPKEGLKPLRLDWIRDRGDIEDYEGRRRNLEDDGKRAIKRGRATKEWRGRKHQPMRAKDH
PITQMWYARHGIITPEMQYVATRENCDVELVRSELAAGRAVMPCNINHPEAEPMIIGSAF
LTKLNANMGNSAVTSSIDEEVEKLTWATKWGADTVMDLSTGNDIHTTREWILRNSPVPIG
TVPMYQALEKVEDDASKLSWELFRDTVIEQCEQGVDYMTIHAGVLLRYVPLTANRVTGIV
SRGGSIMADWCLRHHQESFLYTHFDELCDIFAKYDVAFSLGDGLRPGSLADANDAAQLSE
LMTLGELTERAWAKDVQVMIEGPGHVPFDTVRMNIELEKAVCHNAPFYTLGPLTTDTAPG
YDHITSAIGATEIGRYGTAMLCYVTPKEHLGLPNKDDVKQGVIAYKIACHAADIAKHHPH
AQDRDDAISKARFEFRWLDQFNLSYDPDTAIAFHDETLPAEPAKMAHFCSMCGPKFCSMA
ISQNIRKAFGGEAAQQQIVKEAAAGIDSEALAKAKANVDNDVVSANVLSPEEILAGMDAM
SEKYTAQGGKLYSTAQE