Protein Info for BBR_RS17035 in Bifidobacterium breve UCC2003

Annotation: imidazole glycerol phosphate synthase subunit HisH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00117: GATase
  • amino acids 5 to 211 (207 residues), 56.8 bits, E=1.2e-19
• TIGR01855: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit
  • amino acids 5 to 213 (209 residues), 185.8 bits, E=4e-59

Best Hits

Swiss-Prot: 94% identical to HIS5_BIFLO: Imidazole glycerol phosphate synthase subunit HisH (hisH) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K02501, glutamine amidotransferase [EC: 2.4.2.-] (inferred from 94% identity to bll:BLJ_1326)

Predicted SEED Role

"Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-)" in subsystem Histidine Biosynthesis (EC 2.4.2.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (39/46 steps found)
• L-histidine biosynthesis (10/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.-

Use Curated BLAST to search for 2.4.2.-

Protein Sequence (215 amino acids)

>BBR_RS17035 imidazole glycerol phosphate synthase subunit HisH (Bifidobacterium breve UCC2003)
MTTAVVFDYGFGNVRSMVRALADLGVDTTLTSDYRQALEADGLVVPGVGAFAACMDGLKK
VDGDRVIYDRIRAGRPVLGVCVGEQIMFERGLEHGAHASGIGLIGGSVNLLDADVVPHMG
WDTIEAAPDSVLLNGVENERFYFVHSYAAMEVKPADTARFDIDLQTSTERVSWCTYGRSR
FVAAYERGPLSATQFHPEKSGDAGAQLLKNWIATL