Protein Info for BBR_RS17030 in Bifidobacterium breve UCC2003
Annotation: bifunctional 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase/phosphoribosylanthranilate isomerase PriA
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 98% identical to HIS4_BIFLS: 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) from Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)
KEGG orthology group: K01814, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase [EC: 5.3.1.16] (inferred from 97% identity to blj:BLD_0157)Predicted SEED Role
"Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16) / Acting phosphoribosylanthranilate isomerase (EC 5.3.1.24)" in subsystem Tryptophan synthesis or Histidine Biosynthesis (EC 5.3.1.16, EC 5.3.1.24)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (39/46 steps found)
- superpathway of aromatic amino acid biosynthesis (18/18 steps found)
- superpathway of L-tryptophan biosynthesis (13/13 steps found)
- L-histidine biosynthesis (10/10 steps found)
- L-tryptophan biosynthesis (6/6 steps found)
- superpathway of chorismate metabolism (32/59 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Histidine metabolism
- Phenylalanine, tyrosine and tryptophan biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 5.3.1.24
Use Curated BLAST to search for 5.3.1.16 or 5.3.1.24
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-positive bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (241 amino acids)
>BBR_RS17030 bifunctional 1-(5-phosphoribosyl)-5-((5- phosphoribosylamino)methylideneamino)imidazole-4- carboxamide isomerase/phosphoribosylanthranilate isomerase PriA (Bifidobacterium breve UCC2003) MSLTLLPAVDVRDGKAVRLRQGESGSETDYGSPFEAARTWVEAGAEWIHLVDLDAAFGTG NNRDQLREIVHELGDRVNIELSGGVRDDDSLDAALEAGAARVNIGTAALENPDWTASVIK KYGDRVAVGLDVRGHTLAARGWTKEGGDLFETMKFLDSVGCSRYVVTDVAKDGMMSGPNI GLLREVAERTDAKVTASGGISKLDDLRAIKTLAEIGVDSAILGKSLYARAFTLQEALEVA R