Protein Info for BBR_RS16970 in Bifidobacterium breve UCC2003
Annotation: phosphoglycerate dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 50% identical to SERA_HAEIN: D-3-phosphoglycerate dehydrogenase (serA) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
KEGG orthology group: K00058, D-3-phosphoglycerate dehydrogenase [EC: 1.1.1.95] (inferred from 95% identity to blb:BBMN68_198)MetaCyc: 50% identical to phosphoglycerate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Phosphoglycerate dehydrogenase. [EC: 1.1.1.95]; KETOGLUTREDUCT-RXN [EC: 1.1.1.95, 1.1.1.399]; 1.1.1.399 [EC: 1.1.1.95, 1.1.1.399]
Predicted SEED Role
"D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95)" in subsystem Glycine and Serine Utilization or Pyridoxin (Vitamin B6) Biosynthesis or Serine Biosynthesis (EC 1.1.1.95)
MetaCyc Pathways
- L-serine biosynthesis I (3/3 steps found)
- superpathway of L-serine and glycine biosynthesis I (3/4 steps found)
- L-cysteine biosynthesis IX (Trichomonas vaginalis) (2/3 steps found)
- superpathway of sulfate assimilation and cysteine biosynthesis (4/9 steps found)
- L-glutamate degradation XI (reductive Stickland reaction) (1/7 steps found)
- L-glutamate degradation V (via hydroxyglutarate) (1/10 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.95
Use Curated BLAST to search for 1.1.1.399 or 1.1.1.95
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-positive bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (399 amino acids)
>BBR_RS16970 phosphoglycerate dehydrogenase (Bifidobacterium breve UCC2003) MPKALLLENIHPDAAKSLRDHGFEVETMKGALNEDELIDALEGVDLVGVRSKTNVTSRVL NARPTLSAIGCFCIGTNQVDLDYAGKHGIAVFNAPYSNTRSVVELVICDIICLMRRIPAH THHIKHGLWDKSASGSHEVRGKTLGIIGYGNIGSQLSVLAEALGMRVVFYDIEEKLALGN AHRCSTLNELLEQSDAVTLHVDGRKSNTGFFGEDQFAHMKQDAIFINLSRGFVADLGALK QHLDSGHLSGAAVDVFPVEPKKSGDPFETALANEDNMILTPHIGGSTLEAQESIGHFVSQ RLEDYWSKGSTSLSVNLPQINLTDCKGVCRIAHLHDNLPGVLARVNRVLGEENINISFQS LATEGELGYVVTDVAQKPSPATLEALRSIEGTIRMRVIG