Protein Info for BBR_RS16930 in Bifidobacterium breve UCC2003

Annotation: phospho-N-acetylmuramoyl-pentapeptide- transferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 368 transmembrane" amino acids 6 to 26 (21 residues), see Phobius details amino acids 51 to 73 (23 residues), see Phobius details amino acids 80 to 100 (21 residues), see Phobius details amino acids 120 to 138 (19 residues), see Phobius details amino acids 162 to 183 (22 residues), see Phobius details amino acids 195 to 214 (20 residues), see Phobius details amino acids 235 to 254 (20 residues), see Phobius details amino acids 262 to 282 (21 residues), see Phobius details amino acids 286 to 309 (24 residues), see Phobius details amino acids 337 to 361 (25 residues), see Phobius details TIGR00445: phospho-N-acetylmuramoyl-pentapeptide-transferase" amino acids 19 to 353 (335 residues), 304.3 bits, see alignment E=6.3e-95 PF00953: Glycos_transf_4" amino acids 86 to 282 (197 residues), 112.3 bits, see alignment E=1.2e-36

Best Hits

Swiss-Prot: 94% identical to MRAY_BIFLO: Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K01000, phospho-N-acetylmuramoyl-pentapeptide-transferase [EC: 2.7.8.13] (inferred from 94% identity to bll:BLJ_1298)

Predicted SEED Role

"Phospho-N-acetylmuramoyl-pentapeptide-transferase (EC 2.7.8.13)" in subsystem Peptidoglycan Biosynthesis (EC 2.7.8.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.8.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (368 amino acids)

>BBR_RS16930 phospho-N-acetylmuramoyl-pentapeptide- transferase (Bifidobacterium breve UCC2003)
MIALIIGMLVSLIVTLVGTPLLIRLVHKLHYGQYIRQDGPQSHLVKRGTPTLGGVVINFA
IVLGWGASALYRYLRSGDVPSWSAVLVLFAMLSMGVLGFIDDFAKVRKKQNEGLTVGGKF
IGQFILSTIYAVLALIIPTKSGFPSAQPGMSFIEQPFFSFDFAGRAVAIILFVIWVNFLM
TAWTNAVNLTDGLDGLAAGSSMISFVGFAVIAFWESYHIKGGDHGGYSYAVSDPLDLTII
AICAAVACFGFLWYNSNPASIFMGDTGSLALGGLFAALSIATHTEFLTVILGGLYVMEAM
SDVIQVGYFKMTHKRVFKMAPIHHHFELEGWTETKVVVRFWMIELIFVLLALTIFYCDWV
TRSGLLFS