Protein Info for BBR_RS16845 in Bifidobacterium breve UCC2003

Annotation: bifunctional folylpolyglutamate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 34 to 455 (422 residues), 337.5 bits, E=7.6e-105
• PF08245: Mur_ligase_M
  • amino acids 56 to 289 (234 residues), 50 bits, E=3.7e-17
• PF02875: Mur_ligase_C
  • amino acids 313 to 386 (74 residues), 33.6 bits, E=4.1e-12

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 90% identity to bll:BLJ_1262)

Predicted SEED Role

"Dihydrofolate synthase (EC 6.3.2.12) @ Folylpolyglutamate synthase (EC 6.3.2.17)" (EC 6.3.2.12, EC 6.3.2.17)

MetaCyc Pathways

• tetrahydrofolate biosynthesis I (3/3 steps found)
• folate polyglutamylation (4/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (9/12 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)
• superpathway of tetrahydrofolate biosynthesis (7/10 steps found)
• superpathway of chorismate metabolism (32/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Protein Sequence (533 amino acids)

>BBR_RS16845 bifunctional folylpolyglutamate synthase/dihydrofolate synthase (Bifidobacterium breve UCC2003)
MSFEHPNRNNESMRDVELDIMSRPPEHNTTNLDLDRMNLMLDILGHPEQSFRVIHVTGTN
GKGSTARMAEAICRAYGMRTGLYTSPHLEKVNERIAIDGQQLSDDDFIDVWDQTKDLVAL
VDAKMEEQGKPKMSFFEVLTAMAIWKFADAPVDVAVVEVGMGGLWDATNVLNADAAVIGP
VDMDHMQWLGDTVEQIAGEKAGIIKPNCTAIIGAQPHEEAVMPILEEAAERNHAMLVRDG
YEMTVSDRMAAVGGQVATLTTPNGTYEGVPIAKFGEHQAHNALAALAAAEVVIPVNGPLD
GDLVAEALGSVKIPGRIEQIRTSPTIILDGGHNVNAAEALRKAIEESYDFKQLVGVVAMM
RDKQVEEYLGVLEPILSSVVVTENSWRERVMPADELEKIAVEVFGRDRVIKEPNLPDAIQ
TAVNMVDAEDELGVGYGHGVLVCGSFVTAGDARTMLAEHASPTMQQAMAIHQPAVDLDDA
TDGHGDQPGADTLEDNVSPDDFDVFDVLGLGDAAKNNNADGSSDQDSSAGSDA