Protein Info for BBR_RS16600 in Bifidobacterium breve UCC2003

Annotation: aconitate hydratase AcnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 18 to 898 (881 residues), 1311.2 bits, E=0
• PF00330: Aconitase
  • amino acids 66 to 561 (496 residues), 543.8 bits, E=4.1e-167
• PF00694: Aconitase_C
  • amino acids 691 to 823 (133 residues), 125.5 bits, E=1.8e-40

Best Hits

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 99% identity to bln:Blon_0932)

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• mixed acid fermentation (11/16 steps found)
• nitrogen remobilization from senescing leaves (5/8 steps found)
• partial TCA cycle (obligate autotrophs) (5/8 steps found)
• glyoxylate cycle (3/6 steps found)
• TCA cycle V (2-oxoglutarate synthase) (5/9 steps found)
• ethene biosynthesis V (engineered) (16/25 steps found)
• TCA cycle I (prokaryotic) (5/10 steps found)
• TCA cycle III (animals) (5/10 steps found)
• TCA cycle II (plants and fungi) (4/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (4/9 steps found)
• TCA cycle VI (Helicobacter) (4/9 steps found)
• TCA cycle VII (acetate-producers) (4/9 steps found)
• superpathway of glyoxylate bypass and TCA (5/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (15/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (12/22 steps found)
• reductive TCA cycle I (4/11 steps found)
• reductive TCA cycle II (3/12 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (3/14 steps found)
• methylaspartate cycle (6/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.3

Protein Sequence (899 amino acids)

>BBR_RS16600 aconitate hydratase AcnA (Bifidobacterium breve UCC2003)
MSLRDDQLATLKAAGKDFDYYNIANLDGIDHLPYSLKVLVENLVRNIDGANITDEHVKTL
LDWDPNAEPSHEIQFTPSRVIMQDFTGVPCIVDLATMRDAVKELGGDPEVINPQVQSDMV
IDHSVQIDKYGIANAVQENMDIEYQRNGERYQFLRWGQQAFKNFRVVPPGTGIIHQVNIE
YLAKVVMSKTEANGNTLAYLDSCVGTDSHTTTENGLGVLGWGVGGIEAEAAMLGQPISML
VPRVVGFKLTGSIPEGVTATDVVLTITDMLRKHGVVGKFVEFYGEGIASVPLANRATIGN
MDPEFGSTCGIFPIDNVTLDYLRLTGRSKEQVALVEAYAKANKLWGDASDPDYVEPQYSE
YEELDLGTVVPSIAGPKRPQDRILLSEAKEMFEKTAPAYETEKTVKDPVAVSTDFRGDFD
IENGDVAIASITSCTNTSNPSVMIAAGLIARNAHAKGLKPKPWVKTSLAPGSQVVADYLK
AAGLQDDLDALGYQLVGFGCATCIGNSGPLLPEISEAINTNDLTVTAVLSGNRNFEGRIS
PDVKMNYLASPPLVIAYALAGTMDFDFETQPLGADADGNDVYLKDIWPTNSEVAAVVDGT
VSREMFLKDYASVFDGDHRWKGLDVPEGELFAWNDKSTYVRKQTFFDGMKATPDPVADIH
GARVLALLGDSVTTDHISPAGAFKASSPAGKYLTERGVEPKNFNSYGSRRGNHEIMVRGT
FGNIRLRNQLLASVGEEVTPGGFTYDFLAKKPTTIFEASRDYIDNKVPLVVLAGKEYGTG
SSRDWAAKGTVMLGVKAVITESFERIHRSNLIGMGVLPLQFPAGESYESLGLNGTETYDI
AGVEKLNEGVTPKTVHVTATHEDGSKTEFDAVVRIDTPGEADYYRNGGILQYVLRNLMK