Protein Info for BBR_RS16565 in Bifidobacterium breve UCC2003

Annotation: tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00174: tRNA dimethylallyltransferase
  • amino acids 4 to 288 (285 residues), 227.3 bits, E=1.2e-71
• PF01715: IPPT
  • amino acids 42 to 277 (236 residues), 258.9 bits, E=2.6e-81

Best Hits

Swiss-Prot: 94% identical to MIAA_BIFLD: tRNA dimethylallyltransferase (miaA) from Bifidobacterium longum (strain DJO10A)

KEGG orthology group: K00791, tRNA dimethylallyltransferase [EC: 2.5.1.75] (inferred from 94% identity to bll:BLJ_1192)

Predicted SEED Role

"tRNA dimethylallyltransferase (EC 2.5.1.75)" (EC 2.5.1.75)

MetaCyc Pathways

• cis-zeatin biosynthesis (1/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Zeatin biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.5.1.75

Protein Sequence (327 amino acids)

>BBR_RS16565 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA (Bifidobacterium breve UCC2003)
MKRVVSIVGPTASGKTGLGIAIAKRLAEAGERAEIVNADAYQMYRGMDIGTAKASAEEQA
AVPHHLIDIIEPEDTMTVARFQQLARQTITDLQSRDIRPILVGGSGLYARAAIDDITFPG
TDSDVRKRLEEREKTEGAGVLFDELRDKDPEAAARMDPRNPRRTIRALEVIELTGKPYSA
SLPRYRYVIPSVQIGLDLDRPDLDRRIDLRTKQMYDDGFIKEVERIRPHLGATAVRALGY
QQVIDLLDGIWDVNEAFADIAQKTKRLARKQMGWFGRDPRIHWLQALNPKLVDNAMDIIA
HADAGDYDPIDARADEYTQHHLGDIAA