Protein Info for BBR_RS16475 in Bifidobacterium breve UCC2003

Annotation: type IIA DNA topoisomerase subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 783 PF02518: HATPase_c" amino acids 32 to 136 (105 residues), 52 bits, see alignment E=1.8e-17 PF00204: DNA_gyraseB" amino acids 333 to 530 (198 residues), 149.1 bits, see alignment E=1.9e-47 PF01751: Toprim" amino acids 559 to 663 (105 residues), 61.3 bits, see alignment E=1.6e-20 PF00986: DNA_gyraseB_C" amino acids 712 to 769 (58 residues), 89.1 bits, see alignment 3.4e-29

Best Hits

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (783 amino acids)

>BBR_RS16475 type IIA DNA topoisomerase subunit B (Bifidobacterium breve UCC2003)
MPKEEYGAKDLSVLEGLDAVRKRPGMYIGTTDSQGLMHCLWEIIDNSVDEALAGFCNNIV
VTLHTDGSVEVADNGRGIPVDKEPKTGLSGVEVVLTKLHAGAKFGNSSYNAVGGLHGVGS
SVVNALSSRLDVEVDRDGKTHHMAFHQGHPGVYADADPVNPSPDSPFKRTRKNKPTELEI
IGKVSPKTTGTRIRYWYDPEIFNKTAEFSYEQLIDRVRQTSFLVPGLKITIVDENVPKTN
GADTVGTEPNEDVAVVAESLNANETANATESFSGEDSPLDGFAELSDDASVDGDTEAPDE
EDFSLSSATEVVDGAFGEQPAHPRTVEFLHTGGVKDFVDFLSKGEPISDIWRIQGEGTYK
EETQAVGEGGELHAQEIERTCGVDIALRWVNGYDTTIMSFVNIVETPGGGMHVDGFMNAI
TKQIRKAVEDNARKLKVNLKDSAMKVERDDIQAGLVAVVTARVAEPQFQGQTKDVLGTAQ
VKPIVTRLTDKQFGEMITGSKRGYKEQSARVLEKIVGEMHARIQSRKAKEVTRRKNALES
ASMPAKLSDCQPGNDDVAELFIVEGDSALGTAKAARNAGFQALLPIRGKILNVQKASITQ
MLSNKECAAIIQVVGAGSGQSFDIEQSRYHKIIMMTDADVDGAHIRILLLTLFYRYMRPL
IEHGYVYAAVPPLHRIALTGSHKGEYIYTYSDDELAGKLADLDKKRIGYNDDIQRYKGLG
EMDADQLADTTMDPRTRMLRRIRMEDAAQASEIFNLLMGDEVPPRKQFIVDNADDFDRSK
IDT