Protein Info for BBR_RS16410 in Bifidobacterium breve UCC2003

Annotation: bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 774 TIGR00691: RelA/SpoT family protein" amino acids 48 to 758 (711 residues), 788.1 bits, see alignment E=3.7e-241 PF13328: HD_4" amino acids 48 to 195 (148 residues), 146.9 bits, see alignment E=1.5e-46 PF01966: HD" amino acids 67 to 163 (97 residues), 32.9 bits, see alignment E=2.5e-11 PF04607: RelA_SpoT" amino acids 255 to 365 (111 residues), 145.5 bits, see alignment E=2.7e-46 PF02824: TGS" amino acids 426 to 485 (60 residues), 83.5 bits, see alignment 2.9e-27 PF19296: RelA_AH_RIS" amino acids 501 to 667 (167 residues), 47.3 bits, see alignment E=9.7e-16 PF13291: ACT_4" amino acids 682 to 758 (77 residues), 63.2 bits, see alignment E=1e-20 PF01842: ACT" amino acids 688 to 751 (64 residues), 33.6 bits, see alignment E=9.6e-12

Best Hits

Swiss-Prot: 62% identical to RELA_STRCO: GTP pyrophosphokinase (relA) from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 97% identity to blf:BLIF_1203)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase I" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (774 amino acids)

>BBR_RS16410 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (Bifidobacterium breve UCC2003)
MSEIDGEQYAARKLGCEISADPLNPLEPIKQVCKAHHPGEDLSILDRAYQRAVIQHSVQR
RKSGEPYIIHPLAVSQILADLGMGPIVVAAGLLHDTVEDTDYTLDQCRAEFGDTVAGLVD
GVTKLSQLEVGDSAQAETIRKLVVAMSRDVRTLVVKLADRVHNARTWRYVKTTSAQKKAR
ETLDVYAPLANRLGMNAIKTELEELSFKVLYPKIYNEIVVLVARRAGQRDVYLKQILAEI
NEDLDEQHIKAYVTGRPKDYFSIYQKMIVRGHDFANIYDLVGVRIIVDTIQDCYAALGAV
HARWNPVPGRFKDYIAMPKLNMYQSLHTTVVGPGGKPVEIQIRTWDMHRRAEFGIAAHWK
YKENGQAGRALSSPDKSDRKRDVNNQELSEADNLKWIQQLADWTSETPDSNEFLGSLKED
LGSSEVYVFTPKGKIVSLPAHATPVDFAYAVHTEVGHRTMGARVNGRLVPLDTTLDNGDT
VEILTSKSDTAGPSRDWLSFVKSPKARNKIRQWFSKERRTEAIEEGRDELTRAMRKRNLP
VNALLTTEALIGVADDLNFPNADAVFAAIGDGQISTQNVISHLVKDAGADEVDEEVEQEA
LPLKPVERKSSSSSTGVSVKGVGDVWVKLARCCMPVPGDQIIGFITRNQGVSVHRTDCQN
MIDLKNKQPERVVEVEWTSTKGLFMVKIQVEALDRRNLLSDVTRVLSDHGVNIISGTIAT
GSDRVATSQFSFEMADPQHLNTLLAAVRKIDGVFDVYRLTGAKESAEPRMRKMK