Protein Info for BBR_RS16275 in Bifidobacterium breve UCC2003

Annotation: leucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 987 PF00133: tRNA-synt_1" amino acids 55 to 173 (119 residues), 38.1 bits, see alignment E=1.6e-13 PF09334: tRNA-synt_1g" amino acids 68 to 150 (83 residues), 49 bits, see alignment E=8.3e-17 TIGR00396: leucine--tRNA ligase" amino acids 233 to 984 (752 residues), 679.1 bits, see alignment E=4.6e-208 PF13603: tRNA-synt_1_2" amino acids 307 to 493 (187 residues), 160.7 bits, see alignment E=5.8e-51 PF08264: Anticodon_1" amino acids 835 to 947 (113 residues), 63.9 bits, see alignment E=3.1e-21

Best Hits

Swiss-Prot: 97% identical to SYL_BIFLD: Leucine--tRNA ligase (leuS) from Bifidobacterium longum (strain DJO10A)

KEGG orthology group: K01869, leucyl-tRNA synthetase [EC: 6.1.1.4] (inferred from 97% identity to blb:BBMN68_355)

Predicted SEED Role

"Leucyl-tRNA synthetase (EC 6.1.1.4)" (EC 6.1.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (987 amino acids)

>BBR_RS16275 leucine--tRNA ligase (Bifidobacterium breve UCC2003)
MSDNEKSTQTEEPNFRYNAALAQDIENKWQKIWDEKGTFWAANVNGDLKDGKGRNAEGRT
AYFAMDMFPYPSGKGLHVGHPLGYLASDVVSRYHRMKGENVLHAMGYDAFGLPAEQYAVQ
TGQHPRVTTEANIANMSRQLHRMGLSFDNRRTFATIDPGYVRWTQWIFSRIYDSWYDEDA
TNPSGSKGSARPIAELVAKFESGEKAIPGHESDGKKWADLDQAEQQDILNDFRLAYISKS
PVNWCPGLGTVLANEEVTAEGKSERGNFPVFQRELRQWSMRITKYGHRLIEDLDGINWPE
KVKLMQRNWIGESHGASVHFTVATADGSKDMEIYTTRPDTLFGTTFAVVSPEHHLLENVP
AEWPADVPEDWKGGYANPVEAVKAYRLAAEAKTAKDRVDEAGEKTGLFTGLYATNPITGA
KLPLFTADYVLMDYGTGAIMAVPGGDQRDYDFAVKFGLPVIYTVKPLPESGDDLANYEGK
APFVSHDGIVINSFVEATAAKGDALSLDGLRVDDAIAKVNAWLESAGIGKGTVSYRLRDW
LFSRQRYWGEPFPIVYGEDGTPHLLPDSALPINLPDVPDYEPRTFDPMDAESDPEAPLSR
NEDWVKVELDLGDGVKTYYRDTNTMPNWAGSCWYYMRYIDPTDTKHMVEKDEFDYWMGPN
HNKYSGDEGGVDLYIGGVEHAVLHLLYSRFWHKVLFDLGYVDSAEPFHKLFNQGMIQAYA
YTDDRGQYVPADEVVEGEPDASGEPTFTWNGEHANREFGKMGKSLKNIVTPDYMYENYGA
DTFRLYEMSMGPLDESRPWNTRNVVGGMRFLQRLWRNVVNETTGEVHVSEETPDEKTLKL
LNNTIAEVTAEMEGMRPNTAIAKLIVLNNHLTGLKAVPRAAVEPLILMLAPIAPHICEEM
WNKLGHADSLSAEPWPVADERYVGHDTVTAVVQIKGKVRAKLEVPVDIDPADLEKQARAA
VADRLGGKEPRKVIVKAPKIVSIVPAE