Protein Info for BBR_RS16220 in Bifidobacterium breve UCC2003

Annotation: DNA cytosine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00145: DNA_methylase
  • amino acids 9 to 342 (334 residues), 187.5 bits, E=2.1e-59
• TIGR00675: DNA (cytosine-5-)-methyltransferase
  • amino acids 10 to 340 (331 residues), 130.4 bits, E=5.8e-42

Best Hits

Swiss-Prot: 47% identical to MTD2_HERAU: Modification methylase HgiDII (hgiDIIM) from Herpetosiphon aurantiacus

KEGG orthology group: K00558, DNA (cytosine-5-)-methyltransferase [EC: 2.1.1.37] (inferred from 72% identity to bln:Blon_1145)

Predicted SEED Role

"DNA-cytosine methyltransferase (EC 2.1.1.37)" (EC 2.1.1.37)

KEGG Metabolic Maps

• Methionine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.37

Use Curated BLAST to search for 2.1.1.37

Protein Sequence (349 amino acids)

>BBR_RS16220 DNA cytosine methyltransferase (Bifidobacterium breve UCC2003)
MGKRSKIAAIDLFCGTGGLSLGLKQGGIRVVAGIDNASACSYPYSHNIKAKFIERSVCDV
TGEDLKRLWGQADVRLLAGCAPCQPFSSQRRGADTSHEKSWPLLNEFARLVQETMPDFVT
MENVPRVQYSPIFDEFVARLKQAGYDVTYRVLFGPDYGLPQRRRRLVLLAALNDHIEMPE
PTVGADRYRTVYDAIHDLPSLEAGQKDDDDSLHFARNLSPTNLKRAHASKPGGTWEDWPE
ELRAPCQTRDSGKSFKSFYGRMEWNKPSPTITTQSYNPGAGRFTHPAQDRALTLREASRL
QGFPDSFVFTGPSEKITLSTVGRLIGNAVPPVFGKAIARQFIKTLDDRK