Protein Info for BBR_RS16095 in Bifidobacterium breve UCC2003

Annotation: imidazole glycerol phosphate synthase subunit HisF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00735: imidazoleglycerol phosphate synthase, cyclase subunit
  • amino acids 3 to 256 (254 residues), 365.4 bits, E=6.6e-114
• PF00977: His_biosynth
  • amino acids 6 to 239 (234 residues), 277.4 bits, E=3.5e-86
• PF05690: ThiG
  • amino acids 163 to 232 (70 residues), 23.9 bits, E=1e-08

Best Hits

Swiss-Prot: 99% identical to HIS6_BIFLS: Imidazole glycerol phosphate synthase subunit HisF (hisF) from Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)

KEGG orthology group: K02500, cyclase [EC: 4.1.3.-] (inferred from 98% identity to blo:BL0685)

MetaCyc: 54% identical to imidazole-glycerol-phosphate synthase cycloligase subunit (Thermotoga maritima)
GLUTAMIDOTRANS-RXN [EC: 4.3.2.10]

Predicted SEED Role

"Imidazole glycerol phosphate synthase cyclase subunit (EC 4.1.3.-)" in subsystem Histidine Biosynthesis (EC 4.1.3.-)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (39/46 steps found)
• L-histidine biosynthesis (10/10 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamate and L-glutamine biosynthesis (5/7 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.3.- or 4.3.2.10

Protein Sequence (256 amino acids)

>BBR_RS16095 imidazole glycerol phosphate synthase subunit HisF (Bifidobacterium breve UCC2003)
MSLAVRVIPCLDVDAGRVVKGVHFENLKDAGDPVELAAEYYRQGADEITFLDVTASSSHR
NTMIDVVSRTAEQVFIPMTVGGGVRTPEDVDSLLRCGADKVGVNTAAINDPSLISRVADR
FGNQVLVLSVDARREQGEQHTQSGFEVTTMGGRRSTGIDAIWWVKRAEELGAGEILLNSM
DADGTKEGFDLEMIRAVRKEVKIPIIASGGAGKVEDFPPAIEAGADAVLAASVFHYGILT
IADVKAELKKHGYTVR