Protein Info for BBR_RS15700 in Bifidobacterium breve UCC2003

Annotation: rRNA pseudouridine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01479: S4
  • amino acids 19 to 61 (43 residues), 46 bits, 3.4e-16
• PF00849: PseudoU_synth_2
  • amino acids 81 to 211 (131 residues), 64.5 bits, E=1.3e-21
• TIGR00093: pseudouridine synthase
  • amino acids 84 to 243 (160 residues), 145.4 bits, E=6.1e-47

Best Hits

Swiss-Prot: 51% identical to Y1370_MYCLE: Uncharacterized RNA pseudouridine synthase ML1370 (ML1370) from Mycobacterium leprae (strain TN)

KEGG orthology group: K06178, ribosomal large subunit pseudouridine synthase B [EC: 5.4.99.12] (inferred from 96% identity to blm:BLLJ_1076)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase B (EC 4.2.1.70)" in subsystem Two cell division clusters relating to chromosome partitioning (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Protein Sequence (256 amino acids)

>BBR_RS15700 rRNA pseudouridine synthase (Bifidobacterium breve UCC2003)
MPNAYSRARSAYDDSNAIRLQKLLAQAGFGSRRKCEDMITEGRVEVDGELVTELGTRVDP
HKQQVRVDGSRVRVNPNHVTLALNKPRKVLSAMDDPKGRYTLRDIVGDKYERIFHMGRLD
YDSEGLILMTNDGELSQHVMHPKYEVEKTYIATLEGRISGTVCRRLVTTGVQLDDGWIKL
DHCAIIDSSRDFTMVKVVLHSGKNRIVRRIFGSIGFPVKRLVRTQIGPIKLGELKPGSYR
VLSQAEVRSLSKEVGL