Protein Info for BBR_RS15650 in Bifidobacterium breve UCC2003

Annotation: Holliday junction resolvase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00228: crossover junction endodeoxyribonuclease RuvC
  • amino acids 3 to 157 (155 residues), 125.4 bits, E=9.9e-41
• PF02075: RuvC
  • amino acids 3 to 152 (150 residues), 170.4 bits, E=1.2e-54

Best Hits

Swiss-Prot: 99% identical to RUVC_BIFLO: Crossover junction endodeoxyribonuclease RuvC (ruvC) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K01159, crossover junction endodeoxyribonuclease RuvC [EC: 3.1.22.4] (inferred from 98% identity to blb:BBMN68_417)

MetaCyc: 40% identical to crossover junction endodeoxyribonuclease RuvC (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4)" in subsystem DNA-replication or RuvABC plus a hypothetical (EC 3.1.22.4)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10 or 3.1.22.4

Protein Sequence (194 amino acids)

>BBR_RS15650 Holliday junction resolvase (Bifidobacterium breve UCC2003)
MIILGVDPGLTRCGVGVIEAGEHRRLSFIHVDVVRSSPDITQDLRLLAIYNGLSEKMDRF
APDAVSIERVFAQSNRNTVLGTAQAAGLAMLAAAQRNIPVALHTPTEAKLAITGNGQAQK
IQVERMVTRILNLTKMPTPADAADALALAICHALRPAGALQGGEREQHLTAAQRQWAEAA
QNSARRRMNHDRGM