Protein Info for BBR_RS15535 in Bifidobacterium breve UCC2003

Annotation: phosphoglycerate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 98% identical to PGK_BIFLS: Phosphoglycerate kinase (pgk) from Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)

KEGG orthology group: K00927, phosphoglycerate kinase [EC: 2.7.2.3] (inferred from 97% identity to blo:BL0707)

MetaCyc: 54% identical to plastidic 3-phosphoglycerate kinase (Spinacia oleracea)
Phosphoglycerate kinase. [EC: 2.7.2.3]

Predicted SEED Role

"Phosphoglycerate kinase (EC 2.7.2.3)" in subsystem Calvin-Benson cycle or Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes (EC 2.7.2.3)

MetaCyc Pathways

Bifidobacterium shunt (15/15 steps found)
heterolactic fermentation (16/18 steps found)
homolactic fermentation (11/12 steps found)
glycolysis III (from glucose) (10/11 steps found)
superpathway of glucose and xylose degradation (14/17 steps found)
superpathway of N-acetylneuraminate degradation (17/22 steps found)
glycolysis I (from glucose 6-phosphate) (10/13 steps found)
Entner-Doudoroff pathway I (7/9 steps found)
formaldehyde assimilation III (dihydroxyacetone cycle) (9/12 steps found)
hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
superpathway of anaerobic sucrose degradation (14/19 steps found)
glycolysis II (from fructose 6-phosphate) (8/11 steps found)
glycolysis VI (from fructose) (8/11 steps found)
glycolysis IV (7/10 steps found)
superpathway of glycolysis and the Entner-Doudoroff pathway (12/17 steps found)
sucrose biosynthesis I (from photosynthesis) (6/9 steps found)
Calvin-Benson-Bassham cycle (8/13 steps found)
gluconeogenesis III (7/12 steps found)
ethene biosynthesis V (engineered) (16/25 steps found)
gluconeogenesis I (7/13 steps found)
oxygenic photosynthesis (9/17 steps found)
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (15/26 steps found)
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (12/22 steps found)
superpathway of hexitol degradation (bacteria) (9/18 steps found)
glycerol degradation to butanol (7/16 steps found)
1-butanol autotrophic biosynthesis (engineered) (13/27 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (12/26 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (401 amino acids)

>BBR_RS15535 phosphoglycerate kinase (Bifidobacterium breve UCC2003)
MKTLKDLGDLKGKRILVRADFNVPLDGTTITDDGRIKAALPTIKTLHEKGAKVILMAHLG
RPKGKVVPELSLAPVAARLGELLGTNVPLAKDTYGEDAQAKVAAMNDGDVVLLENVRFNP
EETSKDAAERAAYAKKIAALGEAFVSDGFGVVHRAQGSNYDVAADLPAAAGLLVEKEVKA
LSKATENPERPFTVVLGGSKVSDKLGVIENLLDKADRLVIGGGMVFTFLKAKGYEVGTSL
LEEDQLDKVKGYIATAEKNGVELVLPTDVVVNAGFPAGDTPVAPEVVAADAIPSDKMGLD
IGPDSQKLFHDKIVDSKTVVWNGPMGVFEVPEFAAGTKAVAQGLVDATAAGAFTIVGGGD
SASAVRNLGFPEDGFSHISTGGGASLEFLEGKELPGLKVLE