Protein Info for BBR_RS15450 in Bifidobacterium breve UCC2003

Annotation: dihydroorotate dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 PF01180: DHO_dh" amino acids 26 to 306 (281 residues), 233.7 bits, see alignment E=1.3e-73 TIGR01037: dihydroorotate dehydrogenase family protein" amino acids 27 to 308 (282 residues), 292.1 bits, see alignment E=2.1e-91

Best Hits

Swiss-Prot: 48% identical to PYRDB_ALKMQ: Dihydroorotate dehydrogenase B (NAD(+)), catalytic subunit (pyrD) from Alkaliphilus metalliredigens (strain QYMF)

KEGG orthology group: K00226, dihydroorotate dehydrogenase (fumarate) [EC: 1.3.98.1] (inferred from 96% identity to blf:BLIF_0944)

MetaCyc: 46% identical to dihydroorotate dehydrogenase (catalytic subunit) (Bacillus subtilis subtilis 168)
Orotate reductase (NADH). [EC: 1.3.1.14]

Predicted SEED Role

"Dihydroorotate dehydrogenase, catalytic subunit (EC 1.3.3.1)" in subsystem De Novo Pyrimidine Synthesis (EC 1.3.3.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.3.1, 1.3.98.1

Use Curated BLAST to search for 1.3.1.14 or 1.3.3.1 or 1.3.98.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (323 amino acids)

>BBR_RS15450 dihydroorotate dehydrogenase (Bifidobacterium breve UCC2003)
MSANNQSVQGNDNHINLYEPHVWKHHTKVAGLEWKNLVGTASGTFQYAAVRWFYDVSQLG
AVCTKGASPVPWEGNPSPRTAESPAANINAVGLQNPGIDHYLAEDLPKLKAAGAFVISNV
AGHCDDDYAEVVEKLADSDADMLEINVSCPNVSAGGMSVGADPVALANLMDRLRPLTDKP
MIVKLTPNVTDITVPARAAVEHGADALSMINTLKGMRINIRNGEPIIANVTGGVSGPAVL
PIGLAAVYHTRTVLPDIPIIGLGGIDSGEKALEYLYAGANAVEVGAAALFDPVAPLRVAR
ELDDLLDSRPELAAKLATGQTWR