Protein Info for BBR_RS15420 in Bifidobacterium breve UCC2003

Annotation: bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1060 PF03710: GlnE" amino acids 86 to 335 (250 residues), 131.7 bits, see alignment E=4.9e-42 amino acids 622 to 861 (240 residues), 164.2 bits, see alignment E=5.9e-52 PF08335: GlnD_UR_UTase" amino acids 360 to 459 (100 residues), 56.7 bits, see alignment E=4.2e-19 amino acids 885 to 1029 (145 residues), 48.3 bits, see alignment E=1.7e-16 PF03445: DUF294" amino acids 711 to 794 (84 residues), 24.4 bits, see alignment E=3.7e-09

Best Hits

Swiss-Prot: 98% identical to GLNE_BIFLD: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Bifidobacterium longum (strain DJO10A)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 98% identity to blf:BLIF_0938)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1060 amino acids)

>BBR_RS15420 bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase (Bifidobacterium breve UCC2003)
MESSMFTPSSMDLIRAGLQDLDKARTLFDQLKADDIPDERCAELLSVLAHACDPDVALSN
FVDIVNAMQSSQRDLKHVIPDNNALKRLVTVLGVSDAMGKFMRFKPQLVEAAAVDNCNSH
LFNHTQRRARLLKAVGADPDEPAMPVASKDLAEAATALRSSYRNQLAAIIAQDAVADDPT
SIQPTISRELSDLADAALEGALAIARHETEGSEHVRFTIIGMGKLGAQELNYVSDVDLIY
VVEPVDKDVDHQTLIRVGTKMGTMLQRVCQSAIMGVAEQPLWQIDGGLRPEGKDGALVRV
LSSHKNYYEQWAENWEFQALLKARPVAGDPDLGQAYMDMTRPFVWSASKRKNFVYDCQKM
RKRVEDLIPAPLKDREIKLGRGGLRDVEFTVQMLQLVHGRTDESLRTSNTLDSLQRLSEG
GYVSRKQAVRMSQDYRFERVMEHRQQIWSLKRTHLFPDLGRASVGGLEKKRDIDVDEFNQ
NQELRRLARAFGLHPEELVDKYDDTRREVRHLHLDIYYRPMLPVNAQMENDQIVLSVEAA
QERFESIGFGDPDAAIRHVQALTAGVGRAAKINRIILPAVLQWLGEGQNPDMGLLNWRKL
EENFGTESGYLGFLRDSTSAAQRLCHILSNSRFLGDALNKSVESISWLGDDDNLQARTRG
ALDVQTGSALERFGSNINEFATSMRAMRRYEIERIGLSWMSGVISDSDSLKAMTDVYDAI
IDASLTWAVRHQIAAFGVETAPAGITVIAMGRYGGREVNFSSDADAILIYRPADDADDGQ
ANAFAKKVVEDLRNILQGPTTLEPKIELDLDLRPEGKNGPLVRSYASCEEYYESWASTWE
RQALLRARYAAGDAELARDFLINIADPLRYPTTELTEAELQNIRKLKARMEAERLPRGVR
RERHLKLGKGGLSDVEWTVQLMQLQHASDIKDLRVNGTLEALDVLEAKKLISAIDAIQLR
KAWTLCTAARNGNYLWSGRANQADILPDDIYSLGGIAVYLGYGAHRGQHFENDLLAVMRK
CRDVCQRLFYGQVDEDSQSGGTRMHVLAPRLEHNRRLAQR