Protein Info for BBR_RS15230 in Bifidobacterium breve UCC2003

Annotation: elongation factor 4

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01393: elongation factor 4
  • amino acids 15 to 605 (591 residues), 946.6 bits, E=4.9e-289
• PF00009: GTP_EFTU
  • amino acids 16 to 192 (177 residues), 176 bits, E=1.3e-55
• TIGR00231: small GTP-binding protein domain
  • amino acids 18 to 185 (168 residues), 58 bits, E=1e-19
• PF03144: GTP_EFTU_D2
  • amino acids 216 to 286 (71 residues), 36.2 bits, E=1.4e-12
• PF00679: EFG_C
  • amino acids 409 to 494 (86 residues), 75.1 bits, E=7.7e-25
• PF06421: LepA_C
  • amino acids 498 to 603 (106 residues), 162.9 bits, E=4.4e-52

Best Hits

Swiss-Prot: 99% identical to LEPA_BIFLS: Elongation factor 4 (lepA) from Bifidobacterium longum subsp. infantis (strain ATCC 15697 / DSM 20088 / JCM 1222 / NCTC 11817 / S12)

KEGG orthology group: K03596, GTP-binding protein LepA (inferred from 99% identity to blb:BBMN68_588)

MetaCyc: 54% identical to 30S ribosomal subunit biogenesis factor LepA (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"Translation elongation factor LepA" in subsystem Heat shock dnaK gene cluster extended or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (13/18 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (6/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (4/7 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (9/14 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (5/9 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (4/8 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (626 amino acids)

>BBR_RS15230 elongation factor 4 (Bifidobacterium breve UCC2003)
MVVQHNQPGSTDQSVIRNFCIIAHIDHGKSTVADRILQLSGIVPEREMRDRFLDRMDIEQ
ERGITIKSQAVRVPWTFDGTEYTLGMIDTPGHVDFTYEVSRALAACEGAVLLVDATQGIE
AQTLSNLYMAIDHDLAIIPVLNKIDLPSAEPDKHAEEIAGLIGCEPSDVLRVSGKTGEGV
ADLLDQIVMDVPAPHGDPEAPARALIFDSVYDSYRGIVTYIRMEDGELRDREKVHMMGIG
MTHDPIEIGVISPDMTRTKALGAGEVGYIITGAKDVSQSKVGDTLTSAVRPATEPLPGYR
DPKPMVYAGLFPIDNAQFPELRDALDKLKLNDAALIYTPETSVALGFGFRCGFLGLLHME
IVNERLSREFGLDLIQTAPNVTYDVTAEDGSEHHVTNPSEFPDGKIKKIVEPMVAADIIT
PKEFIGAVMDLCQDHRGIMGTMEYISTERVEMHYRIPLAEIVFDFFDQLKSRTKGYASLD
YHEDGEQSADLVKVDILIQGEKVDAFSAIVHRDKAYSYGVMMTKKLRGLIPRQQFEIPIQ
AAIGSRIIARENIRALRKDVLAKCYGGDITRKRKLLEKQKAGKKRMKMLGHVEVPQEAFI
AALSTGEDSNDRDTKDKIRAAQKTEG