Protein Info for BBR_RS15085 in Bifidobacterium breve UCC2003

Annotation: 3-dehydroquinate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01202: SKI
  • amino acids 13 to 171 (159 residues), 137.5 bits, E=9.2e-44
• PF00465: Fe-ADH
  • amino acids 186 to 354 (169 residues), 33.1 bits, E=5.8e-12
• TIGR01357: 3-dehydroquinate synthase
  • amino acids 187 to 533 (347 residues), 350.9 bits, E=4e-109
• PF13685: Fe-ADH_2
  • amino acids 190 to 360 (171 residues), 43.2 bits, E=8.6e-15
• PF01761: DHQ_synthase
  • amino acids 239 to 504 (266 residues), 308.6 bits, E=6e-96

Best Hits

Swiss-Prot: 92% identical to AROKB_BIFLO: Bifunctional shikimate kinase/3-dehydroquinate synthase (aroB) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K13829, shikimate kinase / 3-dehydroquinate synthase [EC: 2.7.1.71 4.2.3.4] (inferred from 92% identity to blf:BLIF_0778)

Predicted SEED Role

"Shikimate kinase I (EC 2.7.1.71) / 3-dehydroquinate synthase (EC 4.2.3.4)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) or Type IV pilus or Benzoate transport and degradation cluster (EC 2.7.1.71, EC 4.2.3.4)

MetaCyc Pathways

• superpathway of aromatic amino acid biosynthesis (18/18 steps found)
• superpathway of L-tryptophan biosynthesis (13/13 steps found)
• superpathway of L-phenylalanine biosynthesis (10/10 steps found)
• superpathway of L-tyrosine biosynthesis (10/10 steps found)
• chorismate biosynthesis I (7/7 steps found)
• chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
• 3-dehydroquinate biosynthesis I (2/2 steps found)
• chorismate biosynthesis II (archaea) (8/12 steps found)
• superpathway of chorismate metabolism (32/59 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Phenylalanine, tyrosine and tryptophan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.71 or 4.2.3.4

Protein Sequence (540 amino acids)

>BBR_RS15085 3-dehydroquinate synthase (Bifidobacterium breve UCC2003)
MTARPHAVIIGMMGAGKTRVGKEVAHMLRLPFADADVEIEHEVGMKIPAYFDKHGEPAFR
EVEADLIADFLEDFDGIFSLGGGAPMTPSTQEALVDYIAQGGCVVYLDADPKEAMERANR
GGGRPMLNGDANARWKKLFKERDPVFRNVANVHVHTRGLTPQGAAKKVIDMVSQRTVHVD
GAAIEPYDVVIGEGAMNRLVDVLGSKPVRIALIHTQPVQRHSDRARALLRQGGYEVSDIV
IPDAEPGKTITVANRIWERLGNEGFTRSDAVVGLGGGAATDLAGFVAATWMRGVRYVNCP
TSLLAMVDASTGGKTGINTPQGKNLVGSFYTPAGVLADMNALVTLPNDIFIEGLGEVAKS
GFIRDPEILTILERHAAQLRAFDGSTFLGSPLEDVVAELIERTVTVKAYHVSSDLKEKGL
REFLNYGHTLAHAIEQLEHFRWRHGNAVAVGMVYAAELAHLTGHIDQDLVDYHRSLLTSL
GLPTSWNGGSFDDVLALMHRDKKARGNELRFVVLDSIGHVVHLDNPPADAVEEAFRRIQQ