Protein Info for BBR_RS15060 in Bifidobacterium breve UCC2003

Annotation: alanine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00344: alanine--tRNA ligase
  • amino acids 6 to 872 (867 residues), 801.5 bits, E=5.2e-245
• PF01411: tRNA-synt_2c
  • amino acids 6 to 563 (558 residues), 583.6 bits, E=5.9e-179
• PF07973: tRNA_SAD
  • amino acids 662 to 706 (45 residues), 55.2 bits, 8.6e-19
• PF02272: DHHA1
  • amino acids 744 to 887 (144 residues), 66.7 bits, E=4.8e-22

Best Hits

Swiss-Prot: 92% identical to SYA_BIFLO: Alanine--tRNA ligase (alaS) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K01872, alanyl-tRNA synthetase [EC: 6.1.1.7] (inferred from 98% identity to bln:Blon_1602)

Predicted SEED Role

"Alanyl-tRNA synthetase (EC 6.1.1.7)" (EC 6.1.1.7)

MetaCyc Pathways

• tRNA charging (19/21 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (2/8 steps found)
• ergotamine biosynthesis (4/12 steps found)
• enterobactin biosynthesis (3/11 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• bacillibactin biosynthesis (2/12 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• superpathway of chorismate metabolism (32/59 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)
• corallopyronin A biosynthesis (2/30 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Aminoacyl-tRNA biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.7

Protein Sequence (892 amino acids)

>BBR_RS15060 alanine--tRNA ligase (Bifidobacterium breve UCC2003)
MRTADIAKRYLDYFAKHDHLVMPSASLISPNPTTLFTIAGMVPFIPYLLGEQTPPKRRMT
SNQKCVRTLDIDEVGKTTRHGTFFQMLGNFSIGDYFKEEAIHYAWELLTTPQDQGGYGFD
PEKLWVTTFTDDEEARSIWKNEGFDPEHMQIFGMEDNFWTTGGPGPGGPCSEIYVDRGPE
YGRDGGPAADETRFIEIWDLVFENYEVDNVKSKTDLHIVGELAQKNIDTGAGLERLAYLM
QGKQNIYETDEVFPVIEAAERLSGRKYGEDEAADVKFRVVADHVRSALMIMSDGVRPSNV
GRGYVLRRLLRRTVQAMRVLGVTDPVIPELLPVSKEAMVASYPELNDTFHDVSEAAYGEE
DAFRRTLENGIEILDVAVKKAKKTADPVVSGSDAFTLHDTYGFPIELTLEMAADQGVKVD
EAKFRELMAEQKSRARADALKKRHNVDLSVYDDFKKTLAKPIDFLGYTDMSARAKVIGIM
QEGKGSVPAVTGPANVEVILDRTPFYAEAGGQLADQGEILSDDGAVLEVDDVQKPIKDLI
VHQCRLTEGTLVVGAEVNANIDLARRGAIARSHTATHMVHKALREELGPQATQRGSEDAP
NRLRFDFQWSKAPAKSVISAVEERVNDKLRDNLAVTTKEMKFDDAIALGAMHLFGEKYGD
VVRVVSIGEDGWSRELCGGTHVDHVGKIGMVNILSEASIGSGVRRVDAVVGQGAYDFNAR
EHALVSQLSDKLNARPDELAERVNTLLAKLKESDRRLASMYEAQLAAAVPALVEDAKNSA
APVKVAIKNVGHFGAVDALRKTVLDVRGQLGEDAPAVVALAGVNEDDKPMVAVATNEAAR
KAGIKAGDLVRGASKILGGGGGGKPDFAQGGGADASKINEALEALKHEAQKA