Protein Info for BBR_RS14985 in Bifidobacterium breve UCC2003

Annotation: ATP-dependent DNA helicase PcrA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 891 PF00580: UvrD-helicase" amino acids 19 to 314 (296 residues), 275.6 bits, see alignment E=1.4e-85 PF13245: AAA_19" amino acids 23 to 299 (277 residues), 72.6 bits, see alignment E=9.1e-24 PF13361: UvrD_C" amino acids 319 to 658 (340 residues), 263.5 bits, see alignment E=9.6e-82 PF13538: UvrD_C_2" amino acids 599 to 657 (59 residues), 35.8 bits, see alignment 1.5e-12 PF21196: PcrA_UvrD_tudor" amino acids 843 to 890 (48 residues), 42 bits, see alignment 1.8e-14

Best Hits

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 93% identity to bln:Blon_1616)

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (891 amino acids)

>BBR_RS14985 ATP-dependent DNA helicase PcrA (Bifidobacterium breve UCC2003)
MYDEPEVIARSAQELVGDLNPQQAEAVQYRGQALLIGAGAGSGKTRVLTRRIAWILSQFG
AWPSQILAITFTNKAAAEMRERLGSLIGPVAQRMWVSTFHSACVRILRRDGKSIGLKSGF
SIYDSADSERLVKIIATEFNLDIKRYTPRSILGHISDLKNNLTGWKENLAVHAPDFTPGQ
RGYQFGTVGDLEAIYAVIYAEYEHRLALANAVDFDDLIGRTVELLRTDPAVAEYYHHRFR
YILVDEYQDTNHAQYVLVRELAGVDTGEKAIPGSPNAGKSGPAWITVVGDSDQSIYAFRG
ADIRNIQDFEQDFPNAKTIMLEQNYRSTQTILDAANAVISNNEGRKPKKLWTALGKGEPI
VGYAADNAQQEAQWVATEIARLHAEEDIAYSDMAIMYRANAQSRSLEEALINAGLPYQLV
GGTKFYERREIKDALAYLQALVNPDDDVNLRRILNVPKRGLGDRAEGILLAYARERGTSF
FYALMHLDEIQDVPTRTATQLKAFRDLMGELSQFTRAHDAKPSEIVAEVLEKSGLRAELE
KSVDPQDASRLENLSQLQSTAAEFEQNTPDATLSAFLETTALVADSDQLPGEAEDSGKVT
LMTLHTAKGLEYPVVFLTGMEQGTFPHSRSMEDTTELQEERRLAYVGITRAKRRLYVTRA
AVRSQWGQAADMMPSQFLDEIPYSLIGWKRREAGVERMRANWESDGFADDYGGWDDDDFG
GTTFGGSSTFGSRGSGSSYGSRSHYGSSSYGSGSSYGSRSGGSSYGSRSSSSYGSRSRSG
SSYGSGSGSSYGSRSRSSSASGGTRAGKVTTRHTTPKSGSAGSSMPSSKLAKDNGLNISD
FAVGDMISHDQYGLGKVTDAQDKGRNSVITVDFGSAGVKRLMLRVAPIEKL