Protein Info for BBR_RS14595 in Bifidobacterium breve UCC2003

Annotation: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 9 to 429 (421 residues), 613.2 bits, E=9.9e-189
• PF01053: Cys_Met_Meta_PP
  • amino acids 11 to 429 (419 residues), 427.8 bits, E=5.7e-132
• PF00155: Aminotran_1_2
  • amino acids 66 to 225 (160 residues), 26.7 bits, E=6.5e-10
• PF01041: DegT_DnrJ_EryC1
  • amino acids 70 to 189 (120 residues), 30.7 bits, E=4.1e-11
• PF00266: Aminotran_5
  • amino acids 94 to 220 (127 residues), 24 bits, E=3.6e-09

Best Hits

Swiss-Prot: 53% identical to CYSD_SCHPO: Homocysteine synthase (met17) from Schizosaccharomyces pombe (strain 972 / ATCC 24843)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 99% identity to blj:BLD_0674)

MetaCyc: 51% identical to O-acetylhomoserine sulfhydrylase (Leptospira meyeri)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
• L-methionine biosynthesis II (5/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (7/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (7/9 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (6/8 steps found)
• homocysteine and cysteine interconversion (3/4 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (4/6 steps found)
• L-homocysteine biosynthesis (1/2 steps found)
• L-methionine biosynthesis I (3/5 steps found)
• L-methionine biosynthesis III (2/4 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (1/5 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (1/5 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (6/12 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (4/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Protein Sequence (438 amino acids)

>BBR_RS14595 O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (Bifidobacterium breve UCC2003)
MAENNKKYRFETLQLHVGQEQADPATDSRAVPIYATTSYVFHNFDHAEARFGLADPGNIY
GRLTNTTQGVFEDRIAALEGGTAGLAVASGAAAVEYAVRNITQSGDHIVAAKNIYGGTFN
LLRHTLPRDGITTTFVSAENPQEFEDAIQENTKLVYFETFGNPNADLPDFEAISEIAHKH
NLPVIVDNTFATPYLFRPLEHGADVVVESATKFIGGHGTTLGGVIVEGGKFDWNAVPGKF
PTLTEPDPSYHGLNFYEALGGAAFVTRVRAILLRDTGATLSPFAAFLLLQGTETLSLRVE
RHVQNALKVVEYLQTVPEVESVSHPSIEGRPDHALYEKYFPNGAGSIFTFDIKGGKDAAR
VFIDNLHLFSLLANVADAKSLVIHPASTTHSQETLEELEDQGIHQGTIRLSIGTENIEDI
LDDLKGGFEALRASGLAK