Protein Info for BBR_RS14490 in Bifidobacterium breve UCC2003

Annotation: ribonuclease D

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 433 PF01612: DNA_pol_A_exo1" amino acids 25 to 189 (165 residues), 101.3 bits, see alignment E=8.2e-33 PF00570: HRDC" amino acids 236 to 296 (61 residues), 39.3 bits, see alignment E=7.8e-14 PF18305: DNA_pol_A_exoN" amino acids 349 to 429 (81 residues), 87.6 bits, see alignment E=8.3e-29

Best Hits

KEGG orthology group: K03684, ribonuclease D [EC: 3.1.13.5] (inferred from 89% identity to blm:BLLJ_0671)

Predicted SEED Role

"Ribonuclease D (EC 3.1.26.3)" in subsystem Experimental tye or tRNA processing (EC 3.1.26.3)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.1.26.3

Use Curated BLAST to search for 3.1.13.5 or 3.1.26.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (433 amino acids)

>BBR_RS14490 ribonuclease D (Bifidobacterium breve UCC2003)
MSDIHDTEPRLLKEPRGGVPDVTSTIEGYHHVCEALADASGSLAADAERASGFRYGHEDW
LIQCKREGAGIALLDPIALTQSGADWNEFNDAVGDATWILHDSLMDLPGFADLGLQPKAL
FDTEIAARLLGLHRFGLAAVTEHYLGITLAKEHSAADWSYRPLPRDWRNYAALDVEVLIE
LENLMRRDLRAAGKDEWAEEEFTHALSEGLAPRKPHPVPWLRISRITQLSRDPQGLAIAK
SLWQERDKLARQHDIAPSLLLSDSSIIEAATRKPHNAAQFRALRSLNERVRIRTGTEQDK
MFERYAPIQRAVKPKVWKEAIDRAMALHPSQWPSMPAPAPDENGIVNAPRSMRLWQQRHP
DRYDRLQRVRRVITSIAEDTRTPAEMIIKPQILRNLCWVENPAEVDVEEFLIAQGARNWQ
VKLIAPSVSGVIM