Protein Info for BBR_RS14465 in Bifidobacterium breve UCC2003

Annotation: NAD+ synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00795: CN_hydrolase
  • amino acids 7 to 250 (244 residues), 95.5 bits, E=3.4e-31
• PF02540: NAD_synthase
  • amino acids 273 to 539 (267 residues), 203.6 bits, E=2.9e-64
• TIGR00552: NAD+ synthetase
  • amino acids 279 to 541 (263 residues), 157.9 bits, E=1.4e-50

Best Hits

KEGG orthology group: K01950, NAD+ synthase (glutamine-hydrolysing) [EC: 6.3.5.1] (inferred from 94% identity to bln:Blon_1717)

Predicted SEED Role

"NAD synthetase (EC 6.3.1.5) / Glutamine amidotransferase chain of NAD synthetase" in subsystem NAD and NADP cofactor biosynthesis global or NAD regulation (EC 6.3.1.5)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• NAD de novo biosynthesis I (6/6 steps found)
• NAD biosynthesis from 2-amino-3-carboxymuconate semialdehyde (4/4 steps found)
• NAD salvage pathway I (PNC VI cycle) (6/7 steps found)
• NAD de novo biosynthesis III (5/6 steps found)
• NAD de novo biosynthesis IV (anaerobic) (5/6 steps found)
• NAD salvage pathway II (PNC IV cycle) (4/5 steps found)
• NAD salvage pathway V (PNC V cycle) (4/5 steps found)
• NAD salvage (plants) (6/11 steps found)
• NAD de novo biosynthesis II (from tryptophan) (4/9 steps found)
• superpathway of NAD biosynthesis in eukaryotes (6/14 steps found)

KEGG Metabolic Maps

• Glutamate metabolism
• Nicotinate and nicotinamide metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.1.5 or 6.3.5.1

Protein Sequence (565 amino acids)

>BBR_RS14465 NAD+ synthase (Bifidobacterium breve UCC2003)
MTQLRFALAQIDTCVGALDANAAKVLDYSRKAAAGDAQVAIFPEMTLTGYPIEDLALRRT
FRQAAWDKANELAGQLQDEGLGHLFVVVGTVGTDRETSKPRNRLVVLHEGVVWTGYDKHF
LPNYGVFDEFRIFSAGNRSVTLEVNGTTIGVAICEDIWQDGGPVAELATKNIDLLLTING
SPYEEGKTNTRFELAQRRAAEVNAPLIYLNQVGGQDDLVFDGGSFVVDADGTLLERSPMF
MEDLTFWDFDAAADHQTKSTIVAELDPDEEVYTACVLGLKDYMAKNHFNGVTLGLSGGID
SALVAAMAADACGGENVWGISMPSMYSSDGSKDDAADLAANIGAHYEVQPIEPLFVAYQQ
QLDLDGVAAENLQARIRGVIVMASSNSRGLLAVATGNKSELACGYSTIYGDAVGGYAPIK
DLLKTRVWEISRWRNKAAAAGVGIGGLKIVGNEEGNAGIPLKNGVMIPVSSIEKAPSAEL
RPGQKDSDSLPEYALLDKVLAAYIEHAHGRADLLADGFDEATVDTVMRLVDRAEWKRRQY
PLGPKVTALAFGRDRRLPITNAFRE