Protein Info for BBR_RS14410 in Bifidobacterium breve UCC2003

Annotation: phosphoketolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 825 PF09364: XFP_N" amino acids 17 to 381 (365 residues), 615.6 bits, see alignment E=3.8e-189 PF03894: XFP" amino acids 414 to 594 (181 residues), 248.7 bits, see alignment E=4.6e-78 PF09363: XFP_C" amino acids 605 to 802 (198 residues), 224.2 bits, see alignment E=1.8e-70

Best Hits

Swiss-Prot: 84% identical to PHK_BIFA0: Probable phosphoketolase (BLA_1483) from Bifidobacterium animalis subsp. lactis (strain AD011)

KEGG orthology group: K01632, fructose-6-phosphate phosphoketolase [EC: 4.1.2.22] (inferred from 99% identity to bll:BLJ_0768)

MetaCyc: 84% identical to D-xylulose 5-phosphate/D-fructose 6-phosphate phosphoketolase (Bifidobacterium animalis lactis)
Phosphoketolase. [EC: 4.1.2.9]; Fructose-6-phosphate phosphoketolase. [EC: 4.1.2.9, 4.1.2.22]

Predicted SEED Role

"Xylulose-5-phosphate phosphoketolase (EC 4.1.2.9) / Fructose-6-phosphate phosphoketolase (EC 4.1.2.22)" in subsystem Fermentations: Lactate or Pentose phosphate pathway (EC 4.1.2.22, EC 4.1.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.2.22 or 4.1.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (825 amino acids)

>BBR_RS14410 phosphoketolase (Bifidobacterium breve UCC2003)
MTSPVIGTPWKKLNAPVSEESLEGVDKYWRVANYLSIGQIYLRSNPLMKAPFTREDVKHR
LVGHWGTTPGLNFLIGHINRFIADHGQNTVIIMGPGHGGPAGTSQSYLDGTYTETFPKIT
KDEAGLQKFFRQFSYPGGIPSHFAPETPGSIHEGGELGYALSHAYGAIMDNPSLFVPAIV
GDGEAETGPLATGWQSNKLVNPRTDGIVLPILHLNGYKIANPTILSRISDEELHEFFHGM
GYEPYEFVAGFDDEDHMSIHRRFAELWETIWDEICDIKAAAQTDNVHRPFYPMLIFRTPK
GWTCPKYIDGKKTEGSWRAHQVPLASARDTEAHFEVLKNWLESYKPEELFDANGAVKDDV
LAFMPKGELRIGANPNANGGVIRDDLKLPNLEDYEVKEVAEYGHGWGQLEATRTLGAYTR
DIIRNNPRDFRIFGPDETASNRLQASYEVTNKQWDAGYISDEVDEHMHVSGQVVEQLSEH
QMEGFLEAYLLTGRHGIWSSYESFVHVIDSMLNQHAKWLEATVREIPWRKPIASMNLLVS
SHVWRQDHNGFSHQDPGVTSVLLNKCFHNDHVIGIYFATDANMLLAIAEKCYKSTNKINA
IIAGKQPAATWLTLDEARAELAKGAAAWDWASTAKNNDEAEVVLAAAGDVPTQEIMAASD
KLKELGVKFKVVNVADLLSLQSAKENDEALSDEEFADIFTADKPVLFAYHSYAHDVRGLI
YDRPNHDNFNVHGYEEEGSTTTPYDMVRVNRIDRYELTAEALRMIDADKYADKIDELEKF
RDEAFQFAVDKGYDHPDYTDWVYSGVNTDKKGAVTATAATAGDNE