Protein Info for BBR_RS14330 in Bifidobacterium breve UCC2003

Annotation: glycogen debranching enzyme GlgX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 703 TIGR02100: glycogen debranching enzyme GlgX" amino acids 2 to 695 (694 residues), 1019.6 bits, see alignment E=0 PF02922: CBM_48" amino acids 3 to 89 (87 residues), 76.1 bits, see alignment E=2.2e-25 PF00128: Alpha-amylase" amino acids 184 to 289 (106 residues), 41.8 bits, see alignment E=1.1e-14

Best Hits

Swiss-Prot: 60% identical to GLGX_MYCTO: Glycogen operon protein GlgX homolog (glgX) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 96% identity to bll:BLJ_0727)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (703 amino acids)

>BBR_RS14330 glycogen debranching enzyme GlgX (Bifidobacterium breve UCC2003)
MYPLGASYDGAGVNFALYSQVAQKVELCLFDEDDAETRVEMTEQNSYVWHNYIPGLQPGQ
RYGYRVYGPYDPANGLRCNPNKLLLDPYAKAIEGNIDGDESLFSYWFKSPDDNTAMNDLD
SAAHTMKSAVINPYFDWGNDQHPYIPYHDSVIYEAHVRGMTNLNMDVPPDIRGTYAGLAH
PSVIEYLKKLGVTAIELMPIHQFVNDSFLQEKGLSNYWGYNTIGFFAPHNAYSSSGERGE
QVNEFKAMVKAYHHAGMEVILDVVYNHTAEGNHMGPTLSFKGIDNASYYRLVEGDQQHYF
DTTGTGNSLLMRSPHALQLITDSLRYWVTEMHVDGFRFDLAATLARQFQEVDKLSAFFDI
VEQDPIISRVKLIAEPWDLGSGGYQVGGFPSSWSEWNGRYRDTVRDFWRSQPSTLPEFAS
RLMGSSDLYQVNGRRPVASVNFITAHDGFTMNDLVSYNEKHNEANGEGNRDGESNNRSWN
CGVEGPTNIRDVNELRQRQMRNMFSTLLLSQGIPMICGGDEVARTQQGNNNAYCQDNEIS
WTDWNLDKNQEELLAFVSKLIHLRLEHPVLHRRRFFAGREPGDDSNTIPQVEWFDHTGSI
MDMDDWQNTHAFSMMIYLNGSDIPEVDWYGNRMVDNDFILIFNAHYEPIMFTLPDERYGR
KWQLVVDTHNPNGPELSYEAGFMITAQSRSFLMLMSAKKPMGL