Protein Info for BBR_RS14240 in Bifidobacterium breve UCC2003

Annotation: 1,4-alpha-glucan branching protein GlgB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 126 to 747 (622 residues), 865.1 bits, see alignment E=1.4e-264 PF02922: CBM_48" amino acids 165 to 235 (71 residues), 67.3 bits, see alignment E=1.8e-22 PF00128: Alpha-amylase" amino acids 297 to 368 (72 residues), 37.3 bits, see alignment E=3.7e-13 PF02806: Alpha-amylase_C" amino acids 652 to 749 (98 residues), 73.1 bits, see alignment E=2.8e-24

Best Hits

Swiss-Prot: 94% identical to GLGB_BIFLO: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Bifidobacterium longum (strain NCC 2705)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 95% identity to bll:BLJ_0709)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-positive bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (750 amino acids)

>BBR_RS14240 1,4-alpha-glucan branching protein GlgB (Bifidobacterium breve UCC2003)
MANETKIKEDTVTVPVAQSDLDAVSNAEFYNPHGVLGGHLGIGKHADTATIRVLRPLAKS
VTILTQDGEYPMTHEYNGVFVTTVPAAGTKKQPTIPDYRVKTEWESGDVLIEDDPYRYMP
TLGEMDTYLFGEGRHEKLWEALGAHVLRYDDPMGGADGTPGEQVVGTAFSVWAPNAHAVR
VVGNFNAWDGRRHAMRELGSSGVWEIFIPGLGAGENYKFQILNANYVWEMKADPMERQHE
IPPNTASIITESSYEWADDAWMQHRRTTNPHNGPVSIYEVHAGSWKKGLSYRDLATELVD
YVKQEGYTHVEFMPLAQHPFSGSWGYQVTGYYAVDSRLGSPDDFRYLVDKFHQAGIGVIM
DWVPAHFPKDAFALGRFDGTPLYEDPDPLRGEHPDWGTYVFNFGRREVRNFLVANALFWL
EDLHVDALRVDAVSSMLYLDYSREPGQWRPNIYGGRENLEAIDFLKEATATAYKNNPGVM
MIAEESTAWPGITAPTSAGGIGFGLKWNMGWMHDTLEYLHEEPINRKWHHNEITFSMVYA
YSENYVLPISHDEVVYGKGSVYGKMPGNGWQKMAGVRELFAYQWAHPGKKLSFMGNELAQ
WGEWDHDASIDWDCLNWQDHRQVQTMVADLNAFYKANPALWSQDFDPAGFQWLTSDDADH
NTLSFLRIGTKGETLAVVVNFSGEAWSDYQVALPTGGKWTEVFTTDDAKYGGSDIHNGTF
EADAGEYHSRPFSAKITVPALAVVFLKPEN